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Combination of Ternary Electrochemiluminescence System of BNQDs/AgMOG-K2S2O8 and Electrochemiluminescence Resonance Energy Transfer Strategy for Ultrasensitive Immunoassay of Amyloid-β Protein
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-12-15 , DOI: 10.1021/acs.analchem.3c02545 Mengting Sheng 1 , Linying Yu 2 , Yao Peng 1 , Qian Wang 1 , Jianshe Huang 2 , Xiurong Yang 1, 2
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-12-15 , DOI: 10.1021/acs.analchem.3c02545 Mengting Sheng 1 , Linying Yu 2 , Yao Peng 1 , Qian Wang 1 , Jianshe Huang 2 , Xiurong Yang 1, 2
Affiliation
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In this work, based on boron nitride quantum dots (BNQDs) as energy donors and MnO2@MWCNTs-COOH as energy receptors, we designed an efficient electrochemiluminescence resonance energy transfer (ECL-RET) immunosensor for the detection of amyloid-β (Aβ42) protein, a biomarker of Alzheimer’s disease (AD). First, the signal amplification of a ternary ECL system composed of BNQDs (as the ECL emitter), K2S2O8 (as the coreactant), and silver metal–organic gels (AgMOG, as the coreaction accelerator) was realized, and PDDA as stabilizer was added, a strong and stable initial ECL signal was obtained. AgMOG could not only support a large amount of BNQDs and Aβ42 capture antibody (Ab1) through Ag–N bond but also exhibit excellent ECL catalytic performance and enhance the luminescent intensity of BNQDs@PDDA-K2S2O8 system. In addition, due to the broad absorption spectrum of MnO2@MWCNTs-COOH and the extensive overlap with the ECL emission spectrum of BNQDs, the quenching probe Ab2-MnO2@MWCNTs-COOH could be introduced into the ternary system through a sandwich immune response. On this basis, the signal on–off ECL immunosensor was constructed to achieve the ultrasensitive detection of Aβ42 through signal transformation. Under the optimal conditions, the prepared ECL biosensor manifested a wide linear range (10 fg/mL–100 ng/mL) with a detection limit of 2.89 fg/mL and showed excellent stability, selectivity, and repeatability, which provided an effective strategy for the ultrasensitive detection of biomarkers in clinical analysis.
中文翻译：
BNQDs/AgMOG-K2S2O8三元电化学发光体系与电化学发光共振能量转移策略相结合用于淀粉样蛋白-β蛋白的超灵敏免疫分析
在这项工作中，我们基于氮化硼量子点（BNQDs）作为能量供体和MnO 2 @MWCNTs-COOH作为能量受体，设计了一种高效的电化学发光共振能量转移（ECL-RET）免疫传感器，用于检测淀粉样蛋白-β（Aβ） 42 ）蛋白质，阿尔茨海默病（AD）的生物标志物。首先，实现了由BNQDs（作为ECL发射体）、K 2 S 2 O 8 （作为共反应剂）和银金属有机凝胶（AgMOG作为共反应加速剂）组成的三元ECL体系的信号放大，并且添加PDDA作为稳定剂，获得了强而稳定的初始ECL信号。 AgMOG不仅可以通过Ag-N键负载大量BNQDs和Aβ 42捕获抗体(Ab 1 )，而且表现出优异的ECL催化性能，增强BNQDs@PDDA-K 2 S 2 O 8体系的发光强度。此外，由于MnO 2 @MWCNTs-COOH的宽吸收光谱以及与BNQDs的ECL发射光谱的广泛重叠，猝灭探针Ab 2 -MnO 2 @MWCNTs-COOH可以通过夹层引入三元体系中免疫反应。在此基础上构建了信号开关型ECL免疫传感器，通过信号转换实现Aβ 42的超灵敏检测。在最佳条件下，所制备的ECL生物传感器表现出较宽的线性范围（10 fg/mL–100 ng/mL），检测限为2.89 fg/mL，并表现出优异的稳定性、选择性和重复性，为ECL生物传感器的检测提供了有效的策略。临床分析中生物标志物的超灵敏检测。
更新日期：2023-12-15
中文翻译：
BNQDs/AgMOG-K2S2O8三元电化学发光体系与电化学发光共振能量转移策略相结合用于淀粉样蛋白-β蛋白的超灵敏免疫分析
在这项工作中，我们基于氮化硼量子点（BNQDs）作为能量供体和MnO 2 @MWCNTs-COOH作为能量受体，设计了一种高效的电化学发光共振能量转移（ECL-RET）免疫传感器，用于检测淀粉样蛋白-β（Aβ） 42 ）蛋白质，阿尔茨海默病（AD）的生物标志物。首先，实现了由BNQDs（作为ECL发射体）、K 2 S 2 O 8 （作为共反应剂）和银金属有机凝胶（AgMOG作为共反应加速剂）组成的三元ECL体系的信号放大，并且添加PDDA作为稳定剂，获得了强而稳定的初始ECL信号。 AgMOG不仅可以通过Ag-N键负载大量BNQDs和Aβ 42捕获抗体(Ab 1 )，而且表现出优异的ECL催化性能，增强BNQDs@PDDA-K 2 S 2 O 8体系的发光强度。此外，由于MnO 2 @MWCNTs-COOH的宽吸收光谱以及与BNQDs的ECL发射光谱的广泛重叠，猝灭探针Ab 2 -MnO 2 @MWCNTs-COOH可以通过夹层引入三元体系中免疫反应。在此基础上构建了信号开关型ECL免疫传感器，通过信号转换实现Aβ 42的超灵敏检测。在最佳条件下，所制备的ECL生物传感器表现出较宽的线性范围（10 fg/mL–100 ng/mL），检测限为2.89 fg/mL，并表现出优异的稳定性、选择性和重复性，为ECL生物传感器的检测提供了有效的策略。临床分析中生物标志物的超灵敏检测。
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