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Synthesis of Truncated DNA Aptamer and Its Application to an Electrochemical Biosensor Consisting of an Aptamer and a MXene Heterolayer for Yellow Fever Virus
BioChip Journal ( IF 5.5 ) Pub Date : 2023-12-18 , DOI: 10.1007/s13206-023-00133-z
Nayeon Kwon , Siyun Lee , Moonbong Jang , Jin-Ho Lee , Chulhwan Park , Taek Lee

Yellow fever virus (YFV) is an acute infectious virus with high morbidity and mortality risks during the toxic phase. Early diagnosis and suppression are essential because YFV has no precise treatment. With the aim of detecting YFV, we fabricated a highly sensitive electrochemical biosensor comprised with a truncated DNA aptamer/MXene heterolayer. The synthesized DNA aptamer was prepared by systematic evolution of ligands using the exponential enrichment (SELEX) technique, which can specifically detect the YFV NS1 protein. MXenes increase the electrical sensitivity and the possibility of attachment of aptamers by widening the surface area. The aptamer-cutting process which called a truncation process can reduce the production cost of biosensors. The biosensor performance was evaluated using electrochemical methods, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The limit of detection (LOD) was 2.757 pM for YFV diluted in phosphate-buffered saline (PBS) and 2.366 pM for YFV diluted in 10% human serum, proving that the biosensor specifically binds to YFV through selectivity evaluation. This biosensor can be a valuable tool for the early diagnosis of YFV, enabling timely intervention as well as facilitating the control and prevention of yellow fever outbreaks.



中文翻译:

黄热病病毒截短DNA适配体的合成及其在适配体和MXene异质层电化学生物传感器中的应用

黄热病病毒(YFV)是一种急性传染性病毒,在中毒阶段具有很高的发病率和死亡风险。由于 YFV 没有精确的治疗方法,因此早期诊断和抑制至关重要。为了检测 YFV,我们制造了一种由截短的 DNA 适体/MXene 异质层组成的高灵敏度电化学生物传感器。合成的DNA适配体采用指数富集(SELEX)技术通过配体系统进化制备,可特异性检测YFV NS1蛋白。 MXene 通过扩大表面积来增加电灵敏度和适体附着的可能性。适配体切割过程称为截断过程,可以降低生物传感器的生产成本。使用电化学方法、循环伏安法(CV)和电化学阻抗谱(EIS)评估生物传感器的性能。 YFV 在磷酸盐缓冲盐水 (PBS) 中稀释的检测限 (LOD) 为 2.757 pM,YFV 在 10% 人血清中稀释的检测限 (LOD) 为 2.366 pM,通过选择性评估证明该生物传感器与 YFV 特异性结合。这种生物传感器可以成为黄热病早期诊断的宝贵工具,能够及时干预并促进黄热病疫情的控制和预防。

更新日期:2023-12-18
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