Immobilized enzymes exhibit favorable advantages in biocatalysis, such as high operation stability, feasible reusability, and improved organic solvents tolerance. Herein, an immobilized ω-amine transaminase AtATA@MWCNTs-NH₂ is successfully prepared using amino modified multi-walled carbon nanotubes as carrier and glutaraldehyde as crosslinker. Under the optimum immobilization conditions, the activity recovery is 78.7%. Compared with purified enzyme AtATA, AtATA@MWCNTs-NH₂ possesses superior stability, even in harsh conditions (e.g., high temperature, acidic or alkali environment, and different kind of organic solvents). To simplify the separation and extraction of products, we choose methanol (10%, v/v) as the cosolvent, replacing DMSO (20%, v/v) in our previous work, for the catalytic reaction of AtATA@MWCNTs-NH₂. AtATA@MWCNTs-NH₂ can be used for stereoselective synthesis (R)-(+)− 1(1-naphthyl)ethylamine ((R)-NEA) for 15 cycles, with the e.e._p (enantiomeric excess) > 99.5%. The catalytic process of AtATA@MWCNTs-NH₂ achieves cycle production of (R)-NEA using methanol as cosolvent.

固定化酶在生物催化中表现出良好的优势，如操作稳定性高、可重复使用、提高有机溶剂耐受性等。本论文以氨基修饰多壁碳纳米管为载体，戊二醛为交联剂，成功制备了固定化ω-胺转氨酶At ATA@MWCNTs-NH _{2 。}在最佳固定化条件下，活性回收率为78.7%。与纯化的At ATA酶相比，At ATA@MWCNTs-NH ₂即使在恶劣的条件下（例如高温、酸性或碱性环境以及不同种类的有机溶剂）也具有优异的稳定性。为了简化产物的分离和提取，我们选择甲醇（10％，v/v ）作为共溶剂，代替我们之前工作中的DMSO（20％，v/v ），用于At ATA@MWCNTs-NH的催化反应₂ . ATA @MWCNTs-NH ₂可用于立体选择性合成( R )-(+)−1(1-萘基)乙胺(( R )-NEA)15个循环，ee p _（对映体过量）> 99.5% 。At ATA@MWCNTs-NH ₂催化过程以甲醇为共溶剂实现了( R )-NEA的循环生产。