The iron metabolism partners Leptospirillum ferriphilum and Acidiphilium sp. were screened from industrial bioheap site. An integrated multi-stage strategy was proposed to improve chalcolite column bioleaching coupling with synergistical utilization of cellulosic waste such as acid hydrolysate of aquatic plants. L. ferriphilum was used to accelerate the initial iron metabolism, and Acidithiobacillus caldus maintained a lower pH in the middle stage, while Acidiphilium sp. greatly inhibited jarosite passivation in the later stage. Meanwhile, L. ferriphilum (38.3 %) and Acidiphilium sp. (37.0 %) dominated the middle stage, while the abundance of Acidiphilium sp. reached 63.5 % in the later stage. The ferrous, sulfate ion and biomass were improved and the transcriptional levels of some biofilm and morphology related genes were significantly up-regulated. The final Cu²⁺ concentration reached 325.5 mg·L^-1, improved by 43.8 %. Moreover, Canonical Correlation Analysis (CCA) analysis between bioleaching performance, iron/sulfur metabolism and community verified the important role of iron metabolism partners.

铁代谢伙伴是嗜铁钩端螺旋菌 (Leptospirillum ferriphilum)和嗜酸菌 (Acidiphilium sp.)。是从工业生物堆场筛选出来的。提出了一种综合的多阶段策略，以改善铜铜矿柱生物浸出与水生植物酸水解产物等纤维素废物的协同利用的耦合。 L. ferriphilum用于加速初始铁代谢， Acidithiobacillus caldus在中期维持较低的 pH，而Acidiphilium sp.极大地抑制了黄钾铁矾后期的钝化。同时， L. ferriphilum (38.3 %) 和Acidiphilium sp。 (37.0 %) 在中间阶段占主导地位，而Acidiphilium sp 的丰度。后期达到63.5%。亚铁离子、硫酸根离子和生物量得到改善，一些生物膜和形态相关基因的转录水平显着上调。最终Cu ²⁺浓度达到325.5 mg·L ^-1 ，提高了43.8 %。此外，生物浸出性能、铁/硫代谢和群落之间的典型相关分析（CCA）分析验证了铁代谢伙伴的重要作用。