Electrotransfer of nucleic acids and proteins has become crucial in biotechnology for gene augmentation and genome editing. This review explores the applications of electrotransfer in both and scenarios, emphasizing biomedical uses. We provide insights into completed clinical trials and successful instances of nucleic acid and protein electrotransfer into therapeutically relevant cells such as immune cells and stem and progenitor cells. In addition, we delve into emerging areas of electrotransfer where nanotechnology and deep learning techniques overcome the limitations of traditional electroporation.

中文翻译：

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用于核酸和蛋白质递送的电转移


核酸和蛋白质的电转移在基因增强和基因组编辑的生物技术中变得至关重要。本综述探讨了电转移在两种情况下的应用，强调生物医学用途。我们提供有关已完成的临床试验以及将核酸和蛋白质电转移到治疗相关细胞（例如免疫细胞、干细胞和祖细胞）的成功实例的见解。此外，我们还深入研究电转移的新兴领域，其中纳米技术和深度学习技术克服了传统电穿孔的局限性。