3-Acetyl-11-keto-β-boswellic acid (AKBA) is a phytoconstituent derived from extract. It shows anti-rheumatic activity by selectively and non-competitively inhibiting the 5-Lipoxygenase (5-LOX) enzyme. AKBA is utilized for complex nanocarriers for its effective topical delivery at the arthritic site. There is a demand to develop a rapid, sensitive, and economical UV–Visible spectroscopic method for AKBA, which can be utilized in nanoformulation designing and day-to-day analysis of and samples. In this research work, an analytical method was developed for the estimation of AKBA in two solvent systems, methanol and phosphate buffer saline pH 7.4, using a UV–Visible spectrophotometer. Further, the developed method was validated for linearity, range, limit of detection, limit of quantification, accuracy, precision, solution stability, and specificity as per regulatory guidelines. The method exhibited a linearity range of 10–50 µg/mL in both media with the obtained value of the high correlation coefficient (>0.9995). Interday and intraday precision samples showed less than 2 % relative standard deviation (% RSD) that exhibited the reliability of the developed method. The accuracy study reflected the % recovery between 99 and 101 % in both media. Further, the developed method was explored to quantify AKBA in formulated lipid nanocarriers (LNCs) formulation. The methanol method was effectively used to estimate the entrapment efficiency in LNCs and skin retention of AKBA. For the quantification of AKBA in release and permeation samples, the phosphate buffer saline pH 7.4 method was utilized. Results indicated that the method could quantify the AKBA in the presence of different sample matrices with reproducibility in nature. In conclusion, a sensitive, simple, economical, precise, and accurate UV–Visible spectroscopic method was developed and validated to quantify AKBA.

中文翻译：

---

光谱法测定复杂脂质纳米载体和皮肤基质中的 3-乙酰基-11-酮-β-乳香酸


3-乙酰基-11-酮-β-乳香酸 (AKBA) 是一种从提取物中提取的植物成分。它通过选择性和非竞争性抑制 5-脂氧合酶 (5-LOX) 表现出抗风湿活性。 AKBA 用于复杂的纳米载体，可在关节炎部位进行有效的局部递送。需要开发一种快速、灵敏且经济的 AKBA 紫外-可见光谱方法，可用于纳米制剂设计和样品的日常分析。在这项研究工作中，开发了一种使用紫外-可见分光光度计估算两种溶剂系统（甲醇和磷酸盐缓冲盐水 pH 7.4）中 AKBA 的分析方法。此外，根据监管指南，对所开发的方法的线性度、范围、检测限、定量限、准确性、精密度、溶液稳定性和特异性进行了验证。该方法在两种介质中表现出 10–50 µg/mL 的线性范围，并获得高相关系数值 (>0.9995)。日间和日内精密度样品的相对标准偏差 (% RSD) 低于 2%，这表明所开发方法的可靠性。准确性研究反映了两种介质的回收率在 99% 到 101% 之间。此外，还探索了所开发的方法来量化配制的脂质纳米载体 (LNC) 制剂中的 AKBA。甲醇法可有效评估 LNC 中的包封率和 AKBA 的皮肤保留率。为了定量释放和渗透样品中的 AKBA，采用了磷酸盐缓冲盐水 pH 7.4 方法。结果表明，该方法可以在不同样品基质存在的情况下定量 AKBA，并且具有本质上的重现性。 总之，开发并验证了一种灵敏、简单、经济、精确和准确的紫外-可见光谱方法来量化 AKBA。