Photosensitizer-singlet oxygen sensor conjugated silica nanoparticles for photodynamic therapy and bioimaging,Chemical Science

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Photosensitizer-singlet oxygen sensor conjugated silica nanoparticles for photodynamic therapy and bioimaging
Chemical Science ( IF 7.6 ) Pub Date : 2023-12-14 , DOI: 10.1039/d3sc03877g
Jeladhara Sobhanan _{1,

2} , Kenji Ono ₃ , Takuya Okamoto _{1,

4} , Makoto Sawada ₃ , Paul S Weiss ₅ , Vasudevanpillai Biju _{1,

4}

Affiliation

Intracellular singlet oxygen (¹O₂) generation and detection help optimize the outcome of photodynamic therapy (PDT). Theranostics programmed for on-demand phototriggered ¹O₂ release and bioimaging have great potential to transform PDT. We demonstrate an ultrasensitive fluorescence turn-on sensor-sensitizer-RGD peptide-silica nanoarchitecture and its ¹O₂ generation–releasing–storing–sensing properties at the single-particle level or in living cells. The sensor and sensitizer in the nanoarchitecture are an aminomethyl anthracene (AMA)-coumarin dyad and a porphyrin or CdSe/ZnS quantum dots (QDs), respectively. The AMA in the dyad quantitatively quenches the fluorescence of coumarin by intramolecular electron transfer, the porphyrin or QD moiety generates ¹O_2, and the RGD peptide facilitates intracellular delivery. The small size, below 200 nm, as verified by scanning electron microscopy and differential light scattering measurements, of the architecture within the ¹O₂ diffusion length enables fast and efficient intracellular fluorescence switching by the tandem ultraviolet (UV)-visible or visible-near-infrared (NIR) photo-triggering. While the red emission and ¹O₂ generation by the porphyrin are continually turned on, the blue emission of coumarin is uncaged into 230-fold intensity enhancement by on-demand photo-triggering. The ¹O₂ production and release by the nanoarchitecture enable spectro-temporally controlled cell imaging and apoptotic cell death; the latter is verified from cytotoxic data under dark and phototriggering conditions. Furthermore, the bioimaging potential of the TCPP-based nanoarchitecture is examined in vivo in B6 mice.

中文翻译：

用于光动力治疗和生物成像的光敏剂-单线态氧传感器共轭二氧化硅纳米粒子

细胞内单线态氧 ( ¹ O ₂ ) 的生成和检测有助于优化光动力疗法 (PDT) 的结果。用于按需光触发¹ O ₂释放和生物成像的治疗诊断学具有改变 PDT 的巨大潜力。我们展示了超灵敏荧光开启传感器-敏化剂-RGD肽-二氧化硅纳米结构及其在单颗粒水平或活细胞中的¹ O ₂生成-释放-存储-传感特性。纳米结构中的传感器和敏化剂分别是氨甲基蒽（AMA）-香豆素二元体和卟啉或CdSe/ZnS量子点（QD）。二元体中的AMA通过分子内电子转移定量猝灭香豆素的荧光，卟啉或QD部分产生¹ O _{2 ，} RGD肽促进细胞内递送。通过扫描电子显微镜和微分光散射测量验证， ¹ O ₂扩散长度内的结构尺寸小，低于 200 nm，可通过串联紫外 (UV)-可见光或可见光-近光进行快速、高效的细胞内荧光切换。 - 红外 (NIR) 光触发。当卟啉的红光发射和¹ O ₂生成持续开启时，香豆素的蓝光发射通过按需光触发释放至 230 倍的强度增强。纳米结构产生和释放的¹ O ₂能够实现光谱时间控制的细胞成像和细胞凋亡；后者通过黑暗和光触发条件下的细胞毒性数据得到验证。此外，基于 TCPP 的纳米结构的生物成像潜力在 B6 小鼠体内进行了体内检查。

更新日期：2023-12-14

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