Enzymatic catalysis exhibits the merits of high catalytic rates and specificity, whereas a major obstacle that hampers commercialization is the need for expensive nicotinamide adenine dinucleotide (NAD⁺) cofactor; thus the regeneration of NAD⁺ is necessary. Here, we report a fullerene-based photocatalyst (C₆₀-ZnTPP) capable of regenerating NAD⁺ through oxidation of NADH by photogenerated holes, accompanied by simultaneous hydrogen formation. Zinc meso-tetraphenylporphine (ZnTPP) and C₆₀ are combined as a donor–acceptor (D–A) structure with a robust internal electric field (IEF, 5.67 times greater than that of ZnTPP), ensuring ultrafast (∼1 ps) and long-lived charge separation (>3 ns) and transfer, which is conducive to improving the performance of photocatalytic regeneration of NAD⁺. NADH is used as the sole hole sacrificial agent in the system, achieving up to 98.6% NAD⁺ regeneration within 5 h under visible light (≥420 nm) illumination. Equivalent oxidation of ethanol is catalyzed by alcohol dehydrogenase, a key enzyme in human alcohol metabolism, to verify the enzymatic activity of photocatalyzed NAD⁺. This work provides an extended choice of materials available for photocatalytic NAD⁺ regeneration, offering valuable insights into optimizing efficient cofactor regeneration pathways.

酶催化具有催化速率高、特异性强等优点，但其商业化的主要障碍是需要昂贵的烟酰胺腺嘌呤二核苷酸（NAD ⁺）辅因子；^{因此NAD +}的再生是必要的。在这里，我们报道了一种基于富勒烯的光催化剂（C ₆₀ -ZnTPP），能够通过光生空穴氧化NADH并同时形成氢气来再生NAD ^{+ 。}内消旋四苯基卟吩锌 (ZnTPP) 和 C ₆₀结合为供体-受体 (D-A) 结构，具有强大的内部电场（IEF，比 ZnTPP 大 5.67 倍），确保超快 (∼1 ps) 和长距离活的电荷分离（>3 ns）和转移，有利于提高NAD ⁺的光催化再生性能。NADH作为系统中唯一的孔牺牲剂，在可见光（≥420 nm）照射下5小时内实现高达98.6%的NAD ^+再生。通过人体酒精代谢的关键酶乙醇脱氢酶催化乙醇的等价氧化，验证光催化NAD ⁺的酶活性。^{这项工作为光催化 NAD +}再生提供了更多的材料选择，为优化高效辅因子再生途径提供了宝贵的见解。