Electric-field driven uranium extraction from seawater has been proved to greatly enhance the performances of various adsorbents via gathering uranyl ions at the surfaces, but high-concentration interfering cations would also be enriched which generates shielding effects to reduce adsorption capacity. To address this issue, we have fabricated nanofiltration membranes with sieving-adsorption properties by choosing polyamidoxime (PAO) as substrates and polyamide as skin layers. On the one hand, the polyamide skin layers fabricated by interfacial polymerization could effectively separate uranyl ions from other metal ions according to the size-sieving effect since the size of uranyl ions is much larger than most interfering cations. The results demonstrated that the nanofiltration membranes could nearly 100 % reject uranyl ions while the rejections of other metal ions below 30 %. On the other hand, the rejected uranyl ions would be enriched at the surfaces of PAO substrates and followed by adsorption. The increased uranium concentrations are beneficial for both adsorption capacity as well as adsorption rate. Therefore, the adsorption capacity increased by 30 % compared with the pristine PAO adsorbents. This work not only provides a new strategy for the design of high-performance adsorbents, but also could promote the engineering process of uranium extraction from seawater which has an important scientific significance and application value.