Starch from Pulses Has Prebiotic Effects on Healthy Human Donors in an In Vitro Study,ACS Food Science & Technology

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Starch from Pulses Has Prebiotic Effects on Healthy Human Donors in an In Vitro Study
ACS Food Science & Technology ( IF 2.6 ) Pub Date : 2023-12-12 , DOI: 10.1021/acsfoodscitech.3c00483
Oluwatoyin O. Sangokunle _{1,

2,

3} , Shridhar K. Sathe ₃

Affiliation

Pulse starch (PS) contributes to the resistant properties associated with the consumption of pulses. Since starch contributes to the resistant starch (RS) properties of pulse seeds, we hypothesized that PS alone has prebiotic effects. Upper GI digestion was performed on PSs in sodium phosphate buffer (pH 6.9) for 3–5 min, cooled (RT, 30 min), dialyzed (6–8 kDa, 36 h, and 6 h change of water interval), and lyophilized (72 h). Upper GI digestion resulted in 11–13% RS in all the investigated PSs, promoting acetate and butyrate production. Further, 16S sequencing results revealed that all the RSs significantly increased Bacteroidetes while significantly decreased Firmicutes. At the Phyla level, RS from PS significantly promoted Bacteroide, Faecalibacterium, Ruminococcus, and Blautia. Our findings showed that PS has RS from PSs modulating gut microbiota composition, facilitating PS-RS prebiotic potential.

中文翻译：

一项体外研究显示豆类淀粉对健康人类捐赠者具有益生元作用

豆类淀粉 (PS) 有助于抵抗与豆类食用相关的特性。由于淀粉有助于豆类种子的抗性淀粉 (RS) 特性，因此我们假设 PS 单独具有益生元作用。在磷酸钠缓冲液（pH 6.9）中对 PS 进行上消化道消化 3-5 分钟，冷却（RT，30 分钟），透析（6-8 kDa，36 小时，6 小时换水间隔），然后冻干（72 小时）。上消化道消化在所有研究的 PS 中产生 11-13% 的 RS，促进乙酸盐和丁酸盐的产生。此外，16S测序结果显示，所有RS均显着增加了拟杆菌门，同时显着减少了厚壁菌门。在门水平上，PS 中的 RS 显着促进拟杆菌属、粪杆菌属、瘤胃球菌属和布劳特氏菌属的生长。我们的研究结果表明，PS 具有来自 PS 的 RS 调节肠道微生物群组成，促进 PS-RS 益生元潜力。

更新日期：2023-12-12

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