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Sensitive immunoenzyme assay for the detection of antibiotic flumequine in honey
Analytical Methods ( IF 2.7 ) Pub Date : 2023-12-11 , DOI: 10.1039/d3ay01714a
Olga D Hendrickson 1 , Anatoly V Zherdev 1 , Boris B Dzantiev 1
Analytical Methods ( IF 2.7 ) Pub Date : 2023-12-11 , DOI: 10.1039/d3ay01714a
Olga D Hendrickson 1 , Anatoly V Zherdev 1 , Boris B Dzantiev 1
Affiliation
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Fluoroquinolone antibiotics are used to cure and protect bees and apiaries from infections. Consequently, they may contaminate honey and other products of beekeeping. In this study, a highly sensitive immunoenzyme assay (EIA) was for the first time developed for the determination of a fluoroquinolone flumequine (FLU) in honey. The EIA was carried out in an indirect competitive format with colorimetric detection. The analysis was characterized by a low limit of detection of 30 pg mL−1. The polyclonal antibodies used showed no cross-reactivity with 24 other (fluoro)quinolones; the assay was highly specific only toward FLU. Different coating FLU–protein conjugates were tested to achieve the most sensitive competitive immunodetection. A highly simplified and rapid (3–5 min) sample preparation was proposed based on the 100–300 times dilution of honey by a buffer. The developed EIA has been tested to detect FLU in honey of different origins, namely acacia, flower, buckwheat, chestnut, and linden honey. It has been demonstrated that 76.2–115.9% of FLU could be determined by the assay. The versatility, simplicity, and rapidity of the EIA enable us to propose this method as an effective tool to control the contamination of honey.
中文翻译:
灵敏免疫酶法检测蜂蜜中抗生素氟甲喹
氟喹诺酮抗生素用于治疗和保护蜜蜂和养蜂场免受感染。因此,它们可能会污染蜂蜜和其他养蜂产品。在这项研究中,首次开发了一种高灵敏度的免疫酶测定法(EIA)来测定蜂蜜中的氟喹诺酮氟甲喹(FLU)。 EIA 采用比色检测的间接竞争形式进行。该分析的特点是检测下限为30 pg mL -1 。使用的多克隆抗体与 24 种其他(氟)喹诺酮类药物没有交叉反应;该检测仅针对 FLU 具有高度特异性。测试了不同涂层的 FLU-蛋白缀合物,以实现最灵敏的竞争性免疫检测。基于用缓冲液将蜂蜜稀释 100-300 倍,提出了一种高度简化和快速(3-5 分钟)的样品制备方法。开发的 EIA 已经过测试,可检测不同来源的蜂蜜(即金合欢蜜、花蜜、荞麦蜜、栗子蜜和椴树蜜)中的 FLU。已证明该检测可测定 76.2–115.9% 的 FLU。 EIA 的多功能性、简单性和快速性使我们能够提出这种方法作为控制蜂蜜污染的有效工具。
更新日期:2023-12-11
中文翻译:
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灵敏免疫酶法检测蜂蜜中抗生素氟甲喹
氟喹诺酮抗生素用于治疗和保护蜜蜂和养蜂场免受感染。因此,它们可能会污染蜂蜜和其他养蜂产品。在这项研究中,首次开发了一种高灵敏度的免疫酶测定法(EIA)来测定蜂蜜中的氟喹诺酮氟甲喹(FLU)。 EIA 采用比色检测的间接竞争形式进行。该分析的特点是检测下限为30 pg mL -1 。使用的多克隆抗体与 24 种其他(氟)喹诺酮类药物没有交叉反应;该检测仅针对 FLU 具有高度特异性。测试了不同涂层的 FLU-蛋白缀合物,以实现最灵敏的竞争性免疫检测。基于用缓冲液将蜂蜜稀释 100-300 倍,提出了一种高度简化和快速(3-5 分钟)的样品制备方法。开发的 EIA 已经过测试,可检测不同来源的蜂蜜(即金合欢蜜、花蜜、荞麦蜜、栗子蜜和椴树蜜)中的 FLU。已证明该检测可测定 76.2–115.9% 的 FLU。 EIA 的多功能性、简单性和快速性使我们能够提出这种方法作为控制蜂蜜污染的有效工具。