This study evaluated the effects of a multi-strain prebiotics and probiotics on the diet of tambaqui Colossoma macropomum. One hundred and fifty juvenile tambaqui (20.2 ± 3.6 g and 10.32 ± 5.78 cm) were randomly distributed in 15 experimental units with a volume of 80 L and fed for 45 days with a diet containing the symbiotic additive at five inclusion levels (0, 2, 4, 6 and 8 g kg feed^− 1). At the end of the period, growth performance, survival, hemato-biochemical and intestinal parameters, as well as the fish’s resistance to stress were evaluated. The additive did not alter the growth performance, hemato-biochemical and intestinal parameters of the tambaqui. However, the hemato-biochemical parameters of aspartate aminotransferase (AST), plasma protein, hematocrit, glucose, triglycerides and cholesterol showed differences between treatments after the stress challenge. AST increased significantly during challenge and post-challenge. Plasma protein increased significantly during and after the challenge. The hematocrit was highest at 48 h after the challenge and lowest in the 2 g, 6 g and 8 g groups. Glucose was significantly reduced 24 h after the challenge, while triglycerides were lower 24 h and 48 h after the challenge. Cholesterol increased significantly in the challenge. There was an interaction between the factors sampling time and symbiotic concentration for hematocrit and glucose. In hypoxic stress situations, the 2 g concentration was more favorable for the fish. We recommend further studies with 2 g kg feed^− 1 in trials lasting more than 45 days.

本研究评估了多菌株益生元和益生菌对 tambaqui Colossoma macropum饮食的影响。将 150 只幼年 tambaqui（20.2 ± 3.6 g 和 10.32 ± 5.78 cm）随机分布在 15 个体积为 80 L 的实验单元中，并用含有 5 个包含水平（0、2 、4、6 和 8 g kg 饲料^{− 1} )。在此期间结束时，评估鱼的生长性能、存活率、血液生化和肠道参数以及对应激的抵抗力。该添加剂不会改变 tambaqui 的生长性能、血液生化和肠道参数。然而，天冬氨酸转氨酶（AST）、血浆蛋白、血细胞比容、葡萄糖、甘油三酯和胆固醇等血液生化参数在应激挑战后显示出治疗之间的差异。攻击期间和攻击后 AST 显着增加。挑战期间和之后血浆蛋白显着增加。攻击后48小时血细胞比容最高，2g、6g和8g组最低。攻击后24小时血糖显着降低，而攻击后24小时和48小时甘油三酯降低。挑战中胆固醇显着增加。血细胞比容和葡萄糖的采样时间和共生浓度因素之间存在相互作用。在缺氧应激情况下，2克浓度对鱼更有利。我们建议在持续超过 45 天的试验中使用 2 g kg 饲料^{− 1}进行进一步研究。