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Dual Mg-Reinforced PCL Membrane with a Janus Structure for Vascularized Bone Regeneration and Bacterial Elimination
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2023-12-08 , DOI: 10.1021/acsbiomaterials.3c01360 Xianli Wang 1, 2, 3 , Peiqi Shen 1, 2, 3 , Nannan Gu 1, 2, 3 , Yi Shao 1, 2, 3 , Mengmeng Lu 4 , Chunbo Tang 4 , Cheng Wang 1, 2, 3 , Chenglin Chu 1, 2, 3 , Feng Xue 1, 2, 3 , Jing Bai 1, 2, 3, 5
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2023-12-08 , DOI: 10.1021/acsbiomaterials.3c01360 Xianli Wang 1, 2, 3 , Peiqi Shen 1, 2, 3 , Nannan Gu 1, 2, 3 , Yi Shao 1, 2, 3 , Mengmeng Lu 4 , Chunbo Tang 4 , Cheng Wang 1, 2, 3 , Chenglin Chu 1, 2, 3 , Feng Xue 1, 2, 3 , Jing Bai 1, 2, 3, 5
Affiliation
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Commercially available guided bone regeneration (GBR) membranes often exhibit limited mechanical properties or bioactivity, leading to poor performance in repairing bone defects. To surmount this limitation, we developed a Janus structural composite membrane (Mg-MgO/PCL) reinforced by dual Mg (Mg sheets and MgO NPs) by using a combined processing technique involving casting and electrospinning. Results showed that the addition of Mg sheets and MgO NPs enhanced the mechanical properties of the composite membrane for osteogenic space maintenance, specifically tensile strength (from 10.2 ± 1.2 to 50.3 ± 4.5 MPa) and compression force (from 0 to 0.94 ± 0.09 N mm–1), through Mg sheet reinforcement and improved crystallization. The dense cast side of the Janus structure membrane displayed better fibroblast barrier capacity than a single fiber structure; meanwhile, the PCL matrix protected the Mg sheet from severe corrosion due to predeformation. The porous microfibers side supported preosteoblast cell adhesion, enhanced osteogenesis, and angiogenesis in vitro, through the biomimetic extracellular matrix and sustainable Mg2+ release. Furthermore, the Mg-MgO/PCL membrane incorporating 2 wt % MgO NPs exhibited remarkable antimicrobial properties, inducing over 88.75% apoptosis in Staphylococcus aureus. An in vivo experiment using the rat skull defect model (Φ = 5 mm) confirmed that the Mg-MgO/PCL membrane significantly improved new bone formation postsurgery. Collectively, our investigation provides valuable insights into the design of multifunctional membranes for clinical oral GBR application.
中文翻译：
具有 Janus 结构的双镁增强 PCL 膜，用于血管化骨再生和细菌消除
市售的引导骨再生（GBR）膜通常表现出有限的机械性能或生物活性，导致修复骨缺损的性能较差。为了克服这一限制，我们通过使用涉及铸造和静电纺丝的组合加工技术，开发了一种由双镁（镁片和氧化镁纳米粒子）增强的Janus结构复合膜（Mg-MgO/PCL）。结果表明，添加镁片和氧化镁纳米颗粒增强了用于成骨空间维持的复合膜的机械性能，特别是拉伸强度（从 10.2 ± 1.2 到 50.3 ± 4.5 MPa）和压缩力（从 0 到 0.94 ± 0.09 N mm） –1 )，通过镁片强化和改善结晶。 Janus结构膜的致密铸侧比单纤维结构表现出更好的成纤维细胞屏障能力；同时，PCL基体保护镁板免受预变形造成的严重腐蚀。多孔微纤维侧通过仿生细胞外基质和可持续的 Mg 2+释放，支持前成骨细胞粘附、增强成骨作用和体外血管生成。此外，掺入 2 wt% MgO NP 的 Mg-MgO/PCL 膜表现出显着的抗菌特性，诱导金黄色葡萄球菌超过 88.75% 的细胞凋亡。使用大鼠颅骨缺损模型（Φ = 5 mm）进行的体内实验证实，Mg-MgO/PCL 膜可显着改善术后新骨形成。总的来说，我们的研究为临床口腔 GBR 应用的多功能膜的设计提供了宝贵的见解。
更新日期：2023-12-08
中文翻译：
具有 Janus 结构的双镁增强 PCL 膜，用于血管化骨再生和细菌消除
市售的引导骨再生（GBR）膜通常表现出有限的机械性能或生物活性，导致修复骨缺损的性能较差。为了克服这一限制，我们通过使用涉及铸造和静电纺丝的组合加工技术，开发了一种由双镁（镁片和氧化镁纳米粒子）增强的Janus结构复合膜（Mg-MgO/PCL）。结果表明，添加镁片和氧化镁纳米颗粒增强了用于成骨空间维持的复合膜的机械性能，特别是拉伸强度（从 10.2 ± 1.2 到 50.3 ± 4.5 MPa）和压缩力（从 0 到 0.94 ± 0.09 N mm） –1 )，通过镁片强化和改善结晶。 Janus结构膜的致密铸侧比单纤维结构表现出更好的成纤维细胞屏障能力；同时，PCL基体保护镁板免受预变形造成的严重腐蚀。多孔微纤维侧通过仿生细胞外基质和可持续的 Mg 2+释放，支持前成骨细胞粘附、增强成骨作用和体外血管生成。此外，掺入 2 wt% MgO NP 的 Mg-MgO/PCL 膜表现出显着的抗菌特性，诱导金黄色葡萄球菌超过 88.75% 的细胞凋亡。使用大鼠颅骨缺损模型（Φ = 5 mm）进行的体内实验证实，Mg-MgO/PCL 膜可显着改善术后新骨形成。总的来说，我们的研究为临床口腔 GBR 应用的多功能膜的设计提供了宝贵的见解。
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