Anammox activity improved significantly by the cross-fed NO from ammonia-oxidizing bacteria and denitrifying bacteria to anammox bacteria,Water Research

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Anammox activity improved significantly by the cross-fed NO from ammonia-oxidizing bacteria and denitrifying bacteria to anammox bacteria
Water Research ( IF 11.4 ) Pub Date : 2023-12-07 , DOI: 10.1016/j.watres.2023.120986
Sixin Zhang ₁ , Chunrui Li ₁ , Han Lv ₁ , Bin Cui ₁ , Dandan Zhou ₁

Affiliation

Nitric oxide (NO) has been suggested as an obligate intermediate in anaerobic ammonium oxidation (anammox), nitrification and denitrification. At the same time, ammonia-oxidizing bacteria (AOB) and denitrifying bacteria (DNB) are always existed in anammox flora, so what is the role of NO produced from AOB and DNB? Could it accelerate nitrogen removal via the anammox pathway with NO as an electron acceptor? To investigate this hypothesis, nitrogen transforming of an anammox biofilter was analyzed, functional gene expression of anammox bacteria (AnAOB), AOB and DNB were compared, and NO source was verified. For anammox biofilter, anammox contributed to 91.3 % nitrogen removal with only 14.4 % of AnAOB being enriched, while DNB was dominant. Meta-omics analysis and batch test results indicated that AOB could provide NO to AnAOB, and DNB also produced NO via up-regulating nirS/K and down-regulating nor. The activation of the anammox pathway of NH4++NO→N2 caused the downregulation of nirS and nxr in Ca. Kuenenia stuttgartiensis. Additionally, changes in nitrogen transforming pathways affected the electron generation and transport, limiting the carbon metabolism of AnAOB. This study provided new insights into improving nitrogen removal of the anammox system.

中文翻译：

氨氧化菌、反硝化菌与厌氧氨氧化菌交叉投加NO，厌氧氨氧化活性显着提高

一氧化氮 (NO) 被认为是厌氧氨氧化 (anammox)、硝化和反硝化过程中的专性中间体。同时，厌氧氨氧化菌群中始终存在氨氧化菌（AOB）和反硝化菌（DNB），那么AOB和DNB产生的NO有何作用呢？它能否以NO作为电子受体通过厌氧氨氧化途径加速脱氮？为了研究这一假设，分析了厌氧氨氧化生物过滤器的氮转化，比较了厌氧氨氧化细菌（AnAOB）、AOB和DNB的功能基因表达，并验证了NO来源。对于厌氧氨氧化生物过滤器，厌氧氨氧化的脱氮率达到 91.3%，仅富集 14.4% 的 AnAOB，而 DNB 占主导地位。元组学分析和批量测试结果表明，AOB可以向AnAOB提供NO，DNB也通过上调nirS/K和下调nor来产生NO。 NH4++NO→N2厌氧氨氧化途径的激活导致Ca中nirS和nxr的下调。斯图加特库内尼亚。此外，氮转化途径的变化影响了电子的产生和传输，限制了 AnAOB 的碳代谢。这项研究为改善厌氧氨氧化系统的脱氮提供了新的见解。

更新日期：2023-12-07

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