High-Yield Synthesis of Phosphatidylserine in a Well-Designed Mixed Micellar System,Journal of Agricultural and Food Chemistry

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High-Yield Synthesis of Phosphatidylserine in a Well-Designed Mixed Micellar System
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-12-07 , DOI: 10.1021/acs.jafc.3c06584
Chengmei Yin ₁ , Jianan Sun ₁ , Weilong Guo ₁ , Yong Xue ₁ , Haiyang Zhang ₁ , Xiangzhao Mao _{1,

2,

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Affiliation

A sustainable enzymatic system is essential for efficient phosphatidylserine (PS) synthesis in industrial production. Conventional biphasic systems face challenges such as excessive organic solvent usage, enzyme-intensive processes, and increased costs. This study introduces a novel approach using chitin nanofibrils (ChNFs) as an immobilization material for phospholipase D (PLD) in a mixed micellar system stabilized by the food-grade emulsifier sodium deoxycholate (SDC). The immobilized enzyme, ChNF-chiA1, was quickly prepared in a one-step process, eliminating the need for purification. By optimizing the reaction conditions, including l-Ser concentration (1.0 M), SDC concentration (10 mM), reaction time (8 h), and enzyme dosage (1.0 U), a remarkable PS yield of 96.74% was achieved in the solvent-free mixed micellar system. The catalytic efficiency of ChNF-chiA1 surpassed that of the free PLD-chiA1 biphasic system by 6.0-fold. This innovative and green biocatalytic technology offers a reusable solution for the high-value enzymatic synthesis of phospholipids, providing a promising avenue for industrial applications.

中文翻译：

在精心设计的混合胶束系统中高产合成磷脂酰丝氨酸

可持续的酶系统对于工业生产中高效合成磷脂酰丝氨酸（PS）至关重要。传统的双相系统面临着有机溶剂使用过多、酶密集型工艺和成本增加等挑战。本研究介绍了一种新方法，在食品级乳化剂脱氧胆酸钠 (SDC) 稳定的混合胶束系统中，使用几丁质纳米纤维 (ChNF) 作为磷脂酶 D (PLD) 的固定材料。固定化酶 ChNF-chiA1 可通过一步过程快速制备，无需纯化。通过优化反应条件，包括l -Ser浓度（1.0 M）、SDC浓度（10 mM）、反应时间（8 h）和酶用量（1.0 U），溶剂中的PS产率达到了96.74% -自由混合胶束系统。 ChNF-chiA1 的催化效率超过游离 PLD-chiA1 双相系统 6.0 倍。这种创新的绿色生物催化技术为高价值的酶法合成磷脂提供了可重复使用的解决方案，为工业应用提供了一条有前景的途径。

更新日期：2023-12-07

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