<br>体外消化过程中 Ca2+ 诱导的酪蛋白胶束聚集：对负载花青素释放的影响,Food & Function

当前位置： X-MOL 学术 › Food Funct. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

体外消化过程中 Ca2+ 诱导的酪蛋白胶束聚集：对负载花青素释放的影响
Food & Function ( IF 5.1 ) Pub Date : 2023-12-08 , DOI: 10.1039/d3fo03684g
Jinbo Ren _{1,

2} , Minjie Liao ₁ , Kaixin Li ₁ , Fang Chen ₁ , Xiaosong Hu _{1,

2} , Lingjun Ma _{1,

2} , Junfu Ji _{1,

2}

Affiliation

胶体磷酸钙（CCP）赋予胶束酪蛋白（MC）可修饰的结构，这赋予其作为递送载体的潜在优势。然而，以MC作为单壁很难实现核心材料在肠道内的多模式释放。在本研究中，我们利用不同冷冻程度的MC作为壁材料制备了一种基于花青素-酪蛋白的递送系统，以实现花青素作为模型核心在肠道中的控释。结果表明，冷冻可以显着降低CCP水平高达50%。添加外源Ca ²⁺的静态体外模拟消化表明，设计的递送系统在胃道中表现出低花青素释放（15%–35%）。肠道中的释放模式取决于 CCP 解离程度。高解离度和低解离度分别对应于缓慢释放（2小时内从15%到65%）和突发释放（5分钟内从35%到90%）。 WAXS/SAXS分析表明，模拟胃液中固有的外源血清Ca ²⁺和CCP解离诱导的内源血清Ca ²⁺协同参与CCP介导的纳米团簇和大聚集体的重建。 MC的冻结程度决定了内源性血清Ca ²⁺水平，从而影响壁MC的胃聚集行为，最终导致花青素的胃肠道释放行为存在较大差异。

"点击查看英文标题和摘要"

The aggregation of casein micelles induced by Ca2+ during in vitro digestion: effects on the release of loaded anthocyanins

Colloidal calcium phosphate (CCP) confers a modifiable structure to micellar casein (MC), which endows it with potential advantages as a delivery carrier. However, it is difficult to achieve multipattern release of the core material in the intestine with MC as a single wall. In this study, we prepared an anthocyanin–casein-based delivery system utilizing MC with different freezing degrees as the wall material with the objective of achieving the controlled release of anthocyanin as the model core in the intestine. The results showed that freezing could significantly reduce the CCP level up to 50%. Static in vitro simulated digestion with the addition of exogenous Ca²⁺ showed that the designed delivery system exhibited low anthocyanin release (15%–35%) in the gastric tract. The pattern of release in the intestine depended on the CCP dissociation degree. High and low dissociation degrees corresponded to slow release (from 15% to 65% within 2 h) and burst release (from 35% to 90% within 5 min), respectively. WAXS/SAXS analysis revealed that exogenous serum Ca²⁺ inherent in simulated gastric fluid and endogenous serum Ca²⁺ induced by CCP dissociation was synergistically involved in the reconstitution of CCP-mediated nanoclusters and large aggregates. The freezing degree of MC determined the endogenous serum Ca²⁺ level, which influenced the gastric aggregation behavior of wall MC and ultimately led to a fairly different gastrointestinal release behavior of anthocyanins.

更新日期：2023-12-08

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南