<br>立碗藓孢子萌发过程中的蛋白质组可塑性——从干燥阶段到异养生长和光合自养的重建,The Plant Journal

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立碗藓孢子萌发过程中的蛋白质组可塑性——从干燥阶段到异养生长和光合自养的重建
The Plant Journal ( IF 6.2 ) Pub Date : 2023-12-07 , DOI: 10.1111/tpj.16574
Lea Hembach ₁ , Philipp W Niemeyer ₂ , Kerstin Schmitt ₃ , Jaccoline M S Zegers ₄ , Patricia Scholz ₅ , Dennis Brandt ₆ , Janis J Dabisch ₁ , Oliver Valerius ₃ , Gerhard H Braus ₃ , Markus Schwarzländer ₆ , Jan de Vries ₄ , Stefan A Rensing _{7,

8} , Till Ischebeck _{1,

2}

Affiliation

Green Biotechnology, Institute of Plant Biology and Biotechnology (IBBP), University of Münster, 48143, Münster, Germany.
Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences and Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, 37077, Göttingen, Germany.
Department for Molecular Microbiology and Genetics, Genetics and Göttingen Center for Molecular Biosciences (GZMB) and Service Unit LCMS Protein Analytics, Institute for Microbiology, University of Göttingen, 37077, Göttingen, Germany.
Department of Applied Bioinformatics, Göttingen Center for Molecular Biosciences (GZMB) and Campus Institute Data Science (CIDAS), Institute for Microbiology and Genetics, University of Göttingen, 37077, Göttingen, Germany.
Laboratoire Reproduction et Développement des Plantes (RDP), UCB Lyon 1, CNRS, INRAE, Université de Lyon, ENS de Lyon, Lyon, France.
Plant Energy Biology, Institute of Plant Biology and Biotechnology (IBBP), University of Münster, 48143, Münster, Germany.
Plant Cell Biology, Department of Biology, University of Marburg, Marburg, Germany.
BIOSS Centre for Biological Signalling Studies, University of Freiburg, Freiburg, Germany.

苔藓孢子的形成被认为是植物进化的里程碑。它们含有支持干燥耐受性和储存化合物积累的蛋白质网络，这些化合物已经存在于藻类中，也可用于种子和花粉中。此外，发芽孢子必须产生蛋白质，驱动异养植物向自养植物的转变。为了深入了解该蛋白质组的可塑性，我们在苔藓（小立碗藓）孢子萌发以及原丝体和配子体的五个时间点对其进行了研究。与之前发表的拟南芥幼苗蛋白质组数据的比较表明，不仅孢子和种子的蛋白质组在功能上相关，而且萌发孢子和幼苗的蛋白质组也有功能相关。我们观察到干燥耐受性、脂滴蛋白质组组成、休眠控制、β-氧化和乙醛酸循环方面的相似性。然而，也存在显着差异。例如，孢子缺乏任何明显的储存蛋白。此外，我们没有检测到拟南芥种子中主要三酰甘油脂肪酶 SUGAR DEPENDENT1 的同源物。相反，我们发现油体脂肪酶家族的三酰甘油脂肪酶和脂氧合酶是孢子中总体最丰富的蛋白质。这一发现表明了通过苔藓中的氧脂质中间体降解三酰甘油的替代途径。孢子与烟草花粉的比较显示出相似性，例如在耐干燥和耐缺氧方面，但总体发育模式与幼苗建立和孢子萌发的情况并不相符。

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Proteome plasticity during Physcomitrium patens spore germination – from the desiccated phase to heterotrophic growth and reconstitution of photoautotrophy

The establishment of moss spores is considered a milestone in plant evolution. They harbor protein networks underpinning desiccation tolerance and accumulation of storage compounds that can be found already in algae and that are also utilized in seeds and pollen. Furthermore, germinating spores must produce proteins that drive the transition through heterotrophic growth to the autotrophic plant. To get insight into the plasticity of this proteome, we investigated it at five timepoints of moss (Physcomitrium patens) spore germination and in protonemata and gametophores. The comparison to previously published Arabidopsis proteome data of seedling establishment showed that not only the proteomes of spores and seeds are functionally related, but also the proteomes of germinating spores and young seedlings. We observed similarities with regard to desiccation tolerance, lipid droplet proteome composition, control of dormancy, and β-oxidation and the glyoxylate cycle. However, there were also striking differences. For example, spores lacked any obvious storage proteins. Furthermore, we did not detect homologs to the main triacylglycerol lipase in Arabidopsis seeds, SUGAR DEPENDENT1. Instead, we discovered a triacylglycerol lipase of the oil body lipase family and a lipoxygenase as being the overall most abundant proteins in spores. This finding indicates an alternative pathway for triacylglycerol degradation via oxylipin intermediates in the moss. The comparison of spores to Nicotiana tabacum pollen indicated similarities for example in regards to resistance to desiccation and hypoxia, but the overall developmental pattern did not align as in the case of seedling establishment and spore germination.

更新日期：2023-12-07

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