<br>芝麻 (Sesamum indicum) 中壁相关激酶样 (WAKL) 家族的全基因组特征鉴定出参与抗性菜豆 (Macrophhomina Phaseolina) 的 SiWAKL6 基因,BMC Plant Biology

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芝麻 (Sesamum indicum) 中壁相关激酶样 (WAKL) 家族的全基因组特征鉴定出参与抗性菜豆 (Macrophhomina Phaseolina) 的 SiWAKL6 基因
BMC Plant Biology ( IF 4.3 ) Pub Date : 2023-12-07 , DOI: 10.1186/s12870-023-04658-1
Wenqing Yan ₁ , Peilin Hu ₁ , Yunxia Ni ₁ , Hui Zhao ₁ , Xintao Liu ₁ , Hengchun Cao ₂ , Min Jia ₁ , Baoming Tian _{1,

3} , Hongmei Miao _{1,

2,

3,

4} , Hongyan Liu _{1,

3,

4}

Affiliation

芝麻炭腐病是芝麻生产中最严重的真菌病害之一，威胁着芝麻的产量和品质。 WAKL 基因通过感知外部信号并将其传递给细胞内受体，在植物对生物胁迫的反应中发挥重要作用。然而，WAKL基因家族及其在芝麻抗性菜豆中的功能仍缺乏。本研究的目的是解释 WAKL 基因在芝麻对菜豆抗性中的作用。本研究对WAKL基因家族进行了全面研究，在芝麻基因组中鉴定出31个WAKL基因。串联重复事件是 SiWAKL 基因家族扩张的主要因素。系统发育分析表明，芝麻SiWAKL基因家族分为4组。 SiWAKL 基因在不同组织中表现出不同的表达模式。在菜豆胁迫下，大多数SiWAKL基因被显着诱导。值得注意的是，SiWAKL6在抗病品种“政植13号”中被强烈诱导。功能分析表明，SiWAKL6 受水杨酸诱导，而非芝麻中的茉莉酸甲酯。 SiWAKL6在转基因拟南芥植物中的过度表达通过诱导参与水杨酸信号通路的基因表达和重建活性氧稳态来增强其对菜豆的抗性。总而言之，这些结果提供了对 SiWAKL 基因家族功能的更好理解，并表明操纵这些 SiWAKL 基因可以提高植物对菜豆的抗性。这些发现有助于进一步了解 SiWAKL 基因在植物免疫中的功能。

"点击查看英文标题和摘要"

Genome-wide characterization of the wall-associated kinase-like (WAKL) family in sesame (Sesamum indicum) identifies a SiWAKL6 gene involved in resistance to Macrophomina Phaseolina

Sesame charcoal rot caused by Macrophomina phaseolina is one of the most serious fungal diseases in sesame production, and threatens the yield and quality of sesame. WAKL genes are important in the plant response to biotic stresses by sensing and transmitting external signals to the intracellular receptor. However, there is still a lack about the WAKL gene family and its function in sesame resistance to M. phaseolina. The aim of this study was to interpret the roles of WAKL genes in sesame resistance to M. phaseolina. In this study, a comprehensive study of the WAKL gene family was conducted and 31 WAKL genes were identified in the sesame genome. Tandem duplication events were the main factor in expansion of the SiWAKL gene family. Phylogenetic analysis showed that the sesame SiWAKL gene family was divided into 4 groups. SiWAKL genes exhibited different expression patterns in diverse tissues. Under M. phaseolina stress, most SiWAKL genes were significantly induced. Notably, SiWAKL6 was strongly induced in the resistant variety “Zhengzhi 13”. Functional analysis showed that SiWAKL6 was induced by salicylic acid but not methyl jasmonate in sesame. Overexpression of SiWAKL6 in transgenic Arabidopsis thaliana plants enhanced their resistance to M. phaseolina by inducing the expression of genes involved in the salicylic acid signaling pathway and reconstructing reactive oxygen species homeostasis. Taken together, the results provide a better understanding of functions about SiWAKL gene family and suggest that manipulation of these SiWAKL genes can improve plant resistance to M. phaseolina. The findings contributed to further understanding of functions of SiWAKL genes in plant immunity.

更新日期：2023-12-07

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