Pseudomonas aeruginosa and Candida albicans are two important pathogens in public health due to the infections they cause in immunocompromised patients and with hospital stay, increasing morbimortality rates. Three groups of Cu₂O, ZnO, and Ag/Cu₂O nanoparticles were synthesized and characterized physicochemically and confronted to P. aeruginosa and C. albicans to determine their antibacterial effect. Statistical analyses were performed using Analysis of Variance (ANOVA) (p < 0.001). The structures of Cu₂O, ZnO, and Ag/Cu₂O nanoparticles were spherical, sized 6 nm, 10 nm, and 50 nm for Ag, Cu₂, and Zn metals, respectively. Furthermore, a 100% antibacterial and antifungal effect against Pseudomonas aeruginosa and Candida albicans was observed for Cu₂O, ZnO, and Ag/Cu₂O nanoparticles respectively. It is concluded from these findings that the nanoparticles synthesized by biogenic and chemical route had a good size between 6 and 50 nm and that Cu₂O, ZnO, and Ag/Cu₂O nanoparticles presented an excellent antibacterial (100% growth inhibition) effect against P. aeruginosa and C. albicans (p < 0.001) compared to the control.

铜绿假单胞菌和白色念珠菌是公共卫生中的两种重要病原体，因为它们会引起免疫功能低下患者和住院患者的感染，从而增加死亡率。合成了三组Cu ₂ O、ZnO和Ag/Cu ₂ O纳米粒子，并对其进行了物理化学表征，并针对铜绿假单胞菌和白色念珠菌进行了测试，以确定其抗菌效果。使用方差分析 (ANOVA) 进行统计分析 (p < 0.001)。 Cu ₂ O、ZnO 和Ag/Cu ₂ O 纳米颗粒的结构为球形，Ag、Cu ₂和Zn 金属的尺寸分别为6 nm、10 nm 和50 nm。此外，Cu ₂ O、ZnO 和Ag/Cu ₂ O 纳米颗粒分别对铜绿假单胞菌和白色念珠菌具有100% 的抗菌和抗真菌作用。从这些结果可以看出，通过生物和化学途径合成的纳米颗粒具有良好的尺寸（6 至 50 nm），并且 Cu ₂ O、ZnO 和 Ag/Cu ₂ O 纳米颗粒表现出优异的抗菌（100% 生长抑制）效果。与对照相比，针对铜绿假单胞菌和白色念珠菌(p < 0.001)。