During meiosis, cohesin and meiosis-specific proteins organize chromatin into an axis-loop architecture, coordinating homologous synapsis, recombination, and ordered chromosome segregation. However, how the meiotic chromosome axis is assembled and differentiated with meiotic progression remains elusive. Here, we explore the dynamic recruitment of two long arms of the bivalent proteins, LAB-1 and LAB-2, in Caenorhabditis elegans. LAB proteins directly interact with the axis core HORMA complexes and weak interactions contribute to their recruitment. LAB proteins phase separate in vitro, and this capacity is promoted by HORMA complexes. During early prophase, synapsis oppositely regulates the axis enrichment of LAB proteins. After the pachytene exit, LAB proteins switch from a reciprocal localization pattern to a colocalization pattern, and the normal dynamic pattern of LAB proteins is altered in meiotic mutants. We propose that LAB recruitment senses axis differentiation, and phase separation of meiotic structures helps subdomain establishment and accurate segregation of the chromosomes.

中文翻译：

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LAB 蛋白的动态募集可感知秀丽隐杆线虫减数分裂染色体轴的分化


在减数分裂期间，粘连蛋白和减数分裂特异性蛋白将染色质组织成轴环结构，协调同源突触、重组和有序染色体分离。然而，减数分裂染色体轴如何随着减数分裂的进展而组装和分化仍然难以捉摸。在这里，我们探索了秀丽隐杆线虫中二价蛋白 LAB-1 和 LAB-2 的两个长臂的动态募集。 LAB 蛋白直接与轴核心 HORMA 复合物相互作用，弱相互作用有助于它们的募集。 LAB 蛋白在体外发生相分离，这种能力由 HORMA 复合物促进。在前期早期，突触相反地调节 LAB 蛋白的轴富集。粗线期退出后，LAB 蛋白从相互定位模式转变为共定位模式，并且 LAB 蛋白的正常动态模式在减数分裂突变体中发生改变。我们提出 LAB 募集感知轴分化，减数分裂结构的相分离有助于子域的建立和染色体的准确分离。