<br>大戟中新型芥子醇酰基转移酶的鉴定和功能表征。,Journal of Agricultural and Food Chemistry

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大戟中新型芥子醇酰基转移酶的鉴定和功能表征。
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-12-04 , DOI: 10.1021/acs.jafc.3c07127
Fan Wang _{1,

2} , Wanli Zhao ₂ , Wei Lv ₂ , Pirui Li ₂ , Mei Tian ₂ , Shu Xu ₂ , Linwei Li ₂ , Ruiyang Wang ₂ , Fei Liu ₂ , Yu Chen ₂ , Xu Feng _{1,

2}

Affiliation

甲氧基丁香酚是一种源自植物的苯基丙烯化合物，具有多种生物活性。化学合成甲氧基丁香酚存在污染问题，而植物提取则存在收率低、成本高等问题。甲氧基丁香酚的生产可以通过酶法有效解决。本研究通过将山黧豆生长后期转录组数据与密切相关植物的酰基转移酶基因进行同源比对，筛选了山黧豆酰基转移酶基因。结果表明，ElBAHD10与早期报道的ScCFAT和PhCFAT关系最密切，发现ScCFAT和PhCFAT催化松柏醇生成乙酸松柏酯的反应。 ElBAHD10基因从山黧豆中成功克隆，随后在大肠杆菌中表达。纯化的蛋白质ElBAHD10催化芥子醇与乙酰辅酶A和肉桂酰辅酶A的反应，分别形成乙酸芥子酯和肉桂酸芥子酯。相反，粗ElBAHD10蛋白可以催化芥子醇直接生成甲氧基丁香酚。表达ElBAHD10的重组大肠杆菌菌株通过全细胞转化产生甲氧基丁香酚。这项研究为通过生物合成方法生产甲氧基丁香酚提供了见解并奠定了基础。

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Identification and Functional Characterization of a Novel Sinapyl Alcohol Acyltransferase from Euphorbia lathyris L.

Methoxyeugenol is a phenylpropene compound derived from plants and has various bioactivities. The chemical synthesis of methoxyeugenol is accompanied by pollution issues, whereas extraction from plants is associated with problems such as low yield and high cost. The production of methoxyeugenol can be effectively addressed through an enzymatic approach. In this study, the acyltransferase genes of Euphorbia lathyris L. were screened by homologous alignment of the transcriptome data of E. lathyris in the late growth stage and the acyltransferase genes of the closely related plant species. The results showed that ElBAHD10 had the closest relationship with earlier reported ScCFAT and PhCFAT, which were found to catalyze the reaction of coniferyl alcohol to generate coniferyl acetate. The ElBAHD10 gene was successfully cloned from E. lathyris and subsequently expressed in Escherichia coli. The purified protein ElBAHD10 catalyzed the reaction of sinapyl alcohol with acetyl CoA and cinnamoyl CoA to form sinapyl acetate and sinapyl cinnamate, respectively. In contrast, the crude ElBAHD10 protein could catalyze sinapyl alcohol to directly generate methoxyeugenol. The recombinant E. coli strain expressing ElBAHD10 produced methoxyeugenol through whole-cell transformation. This study provides insights and lays the foundation for methoxyeugenol production through biosynthetic approaches.

更新日期：2023-12-04

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