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DNA Aptamer Binding Octapeptide Repeat Region of Cellular Prion Protein
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-12-04 , DOI: 10.1021/acs.analchem.3c04557
Jing Sheng 1, 2 , Nan Zhang 1 , Zhenhao Long 1, 2 , Xiangru Zhang 1, 2 , Shuang Zu 3, 4 , Xiangjun Liu 1, 2 , Dihua Shangguan 1, 2, 3
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-12-04 , DOI: 10.1021/acs.analchem.3c04557
Jing Sheng 1, 2 , Nan Zhang 1 , Zhenhao Long 1, 2 , Xiangru Zhang 1, 2 , Shuang Zu 3, 4 , Xiangjun Liu 1, 2 , Dihua Shangguan 1, 2, 3
Affiliation
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Cellular prion protein (PrPC) is highly expressed in a variety of tumor cells and plays a crucial role in neurodegenerative diseases. Its N-terminal domain contains a conserved octapeptide (PHGGGWGQ) repeat sequence. The number of repeats has been correlated with the species as well as the development of associated diseases. Herein, PrPC was identified to be the molecular target of a high-affinity DNA aptamer HA5-68 obtained by cell-SELEX. Aptamer HA5-68 was further optimized to two short sequences (HA5-40-1 and HA5-40-2), and its binding site to PrPC was identified to be located in the loop–stem–loop region of the head of its secondary structure. HA5 series aptamers were demonstrated to bind the octapeptide repeat region of PrPC, as well as the synthesized peptides containing different numbers of octapeptide repeats. The PrPC expression on 42 cell lines was measured by using aptamer HA5-68 as a molecular probe. The clear understanding of the molecular structure and binding mechanism of this set of aptamers will provide information for the design of diagnostic methods and therapeutic drugs targeting PrPC.
中文翻译:
DNA适体结合细胞朊病毒蛋白的八肽重复区域
细胞朊病毒蛋白(PrP C )在多种肿瘤细胞中高表达,在神经退行性疾病中发挥着至关重要的作用。其 N 末端结构域包含保守的八肽 (PHGGGWGQ) 重复序列。重复次数与物种以及相关疾病的发展相关。在此,PrP C被鉴定为通过 cell-SELEX 获得的高亲和力 DNA 适体 HA5-68 的分子靶标。适体HA5-68进一步优化为两条短序列(HA5-40-1和HA5-40-2),其与PrP C的结合位点被鉴定位于其头部的环-茎-环区域。二级结构。 HA5系列适体被证明能够结合PrP C的八肽重复区域,以及含有不同数量的八肽重复的合成肽。使用适配体HA5-68作为分子探针测量42个细胞系上PrP C的表达。清楚地了解这组适体的分子结构和结合机制将为设计针对PrP C的诊断方法和治疗药物提供信息。
更新日期:2023-12-04
中文翻译:
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DNA适体结合细胞朊病毒蛋白的八肽重复区域
细胞朊病毒蛋白(PrP C )在多种肿瘤细胞中高表达,在神经退行性疾病中发挥着至关重要的作用。其 N 末端结构域包含保守的八肽 (PHGGGWGQ) 重复序列。重复次数与物种以及相关疾病的发展相关。在此,PrP C被鉴定为通过 cell-SELEX 获得的高亲和力 DNA 适体 HA5-68 的分子靶标。适体HA5-68进一步优化为两条短序列(HA5-40-1和HA5-40-2),其与PrP C的结合位点被鉴定位于其头部的环-茎-环区域。二级结构。 HA5系列适体被证明能够结合PrP C的八肽重复区域,以及含有不同数量的八肽重复的合成肽。使用适配体HA5-68作为分子探针测量42个细胞系上PrP C的表达。清楚地了解这组适体的分子结构和结合机制将为设计针对PrP C的诊断方法和治疗药物提供信息。