This study aimed to develop and validate an UHPLC-DAD method for quantifying oleandrin in dried leaves and assessing its concentrations in samples from Eastern Algeria.

For this purpose, a total of 100 leaf samples were collected from wild and cultivated shrubs across five provinces in Eastern Algeria during the flowering period. The UHPLC-DAD system was used to identify and quantify oleandrin. Chromatographic separation was performed using a Kinetex C18 Polar column (100 x 2.1 mm, 2.6 μm) from Phenomenex®. The flow rate was of 0.6 mL/min, and the detection wavelength was set at 220 nm.

The mean oleandrin amount in dried leaf samples collected from the entire region was found to be 0.12 ± 0.12 mg/g. The analysis revealed statistically significant differences among the provinces and between wild and cultivated plants. These differences highlight potential disparities in the risk of acute poisoning in case of ingestion. However, no significant differences were observed among plants with different flower colors. Furthermore, no correlation was observed between the mean oleandrin levels and the meteorological parameters recorded during the annual survey of the harvest year, including temperature, humidity, sunshine, and cumulative rainfall. There was also no correlation between the mean, median, and 90th percentile oleandrin levels and the elevation of the collection sites.

本研究旨在开发和验证一种 UHPLC-DAD 方法，用于定量干叶中的夹竹桃苷并评估其在阿尔及利亚东部样品中的浓度。

为此，我们在阿尔及利亚东部五个省份的野生和栽培灌木中采集了总共 100 个开花期的叶子样本。UHPLC-DAD 系统用于鉴定和定量夹竹桃苷。使用 Phenomenex® 的 Kinetex C18 Polar 柱（100 x 2.1 mm，2.6 μm）进行色谱分离。流速为0.6 mL/min，检测波长设置为220 nm。

从整个地区采集的干叶样品中夹竹桃苷的平均含量为 0.12 ± 0.12 mg/g。分析显示各省之间以及野生植物和栽培植物之间存在统计显着差异。这些差异凸显了摄入后急性中毒风险的潜在差异。然而，不同花色的植物之间没有观察到显着差异。此外，夹竹桃苷的平均含量与收获年年度调查期间记录的气象参数（包括温度、湿度、日照和累积降雨量）之间没有观察到相关性。夹竹桃苷的平均值、中值和第 90 个百分位水平与收集地点的海拔之间也没有相关性。