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Discovery of a novel potential polyphosphate accumulating organism without denitrifying phosphorus uptake function in an enhanced biological phosphorus removal process
Science of the Total Environment ( IF 8.2 ) Pub Date : 2023-12-02 , DOI: 10.1016/j.scitotenv.2023.168952 Yiming Zhao 1 , Zhengyu Zhu 1 , Xuyang Chen 1 , Yongmei Li 2
Science of the Total Environment ( IF 8.2 ) Pub Date : 2023-12-02 , DOI: 10.1016/j.scitotenv.2023.168952 Yiming Zhao 1 , Zhengyu Zhu 1 , Xuyang Chen 1 , Yongmei Li 2
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Enhanced biological phosphorus removal (EBPR) is an effective process for phosphorus removal from wastewater. In this study, two lab-scale sequencing batch reactors (SBR) were used to perform EBPR process, in which genus Propioniciclava was unexpectedly accumulated and its relative abundance was over 70 %. A series of tests were conducted to explore the role of Propioniciclava in the two EBPR systems. The two systems performed steadily throughout the study, and the phosphorus removal efficiencies were 96.6 % and 93.5 % for SBR1 and SBR2, respectively. The stoichiometric analysis related to polyphosphate accumulating organisms (PAOs) indicated that polyphosphate accumulating metabolism (PAM) was achieved in the anaerobic phase. It appeared that the Propioniciclava- dominated systems could not perform denitrifying phosphorus removal. Instead, phosphorus was released under anoxic conditions without carbon sources. According to the genomic information from Integrated Microbial Genomes (IMG) database, Propioniciclava owns ppk1 , ppk2 and ppx genes that are associated with phosphorus release and uptake functions. By phylogenetic investigation of communities by reconstruction of unobserved states 2 (PICRUSt2) analysis, the abundance of genes related to phosphorus metabolism was much higher than that of genes related to denitrification. Therefore, Propioniciclava was presumed to be a potential PAO without denitrifying phosphorus uptake function. In addition to Propioniciclava , Tessaracoccus and Thiothrix were also enriched in both systems. Overall, this study proposes a novel potential PAO and broadens the understanding of EBPR microbial communities.
中文翻译：
在强化生物除磷过程中发现一种新型潜在聚磷酸盐积累生物，不具有反硝化吸磷功能
强化生物除磷（EBPR）是一种有效的废水除磷工艺。在本研究中，使用两个实验室规模的序批式反应器（SBR）进行EBPR过程，其中Propioniciclava属意外积累，其相对丰度超过70%。进行了一系列测试来探索丙酸克拉瓦在两个 EBPR 系统中的作用。整个研究过程中，两个系统表现稳定，SBR1和SBR2的除磷效率分别为96.6%和93.5%。与聚磷酸盐积累生物（PAO）相关的化学计量分析表明，聚磷酸盐积累代谢（PAM）是在厌氧阶段实现的。看来以丙酸为主的系统不能进行反硝化除磷。相反，磷是在没有碳源的缺氧条件下释放的。根据综合微生物基因组（IMG）数据库的基因组信息，Propioniciclava拥有与磷释放和吸收功能相关的ppk1、ppk2和ppx基因。通过重建未观察状态2（PICRUSt2）分析对群落进行系统发育调查，与磷代谢相关的基因丰度远高于与反硝化相关的基因丰度。因此，推测Propioniciclava是一种潜在的PAO，不具有反硝化吸磷功能。除了 Propioniciclava 之外，Tessaracaccus 和 Thiothrix 也在两个系统中富集。总的来说，这项研究提出了一种新型的潜在 PAO，并拓宽了对 EBPR 微生物群落的理解。
更新日期：2023-12-02
中文翻译：
在强化生物除磷过程中发现一种新型潜在聚磷酸盐积累生物，不具有反硝化吸磷功能
强化生物除磷（EBPR）是一种有效的废水除磷工艺。在本研究中，使用两个实验室规模的序批式反应器（SBR）进行EBPR过程，其中Propioniciclava属意外积累，其相对丰度超过70%。进行了一系列测试来探索丙酸克拉瓦在两个 EBPR 系统中的作用。整个研究过程中，两个系统表现稳定，SBR1和SBR2的除磷效率分别为96.6%和93.5%。与聚磷酸盐积累生物（PAO）相关的化学计量分析表明，聚磷酸盐积累代谢（PAM）是在厌氧阶段实现的。看来以丙酸为主的系统不能进行反硝化除磷。相反，磷是在没有碳源的缺氧条件下释放的。根据综合微生物基因组（IMG）数据库的基因组信息，Propioniciclava拥有与磷释放和吸收功能相关的ppk1、ppk2和ppx基因。通过重建未观察状态2（PICRUSt2）分析对群落进行系统发育调查，与磷代谢相关的基因丰度远高于与反硝化相关的基因丰度。因此，推测Propioniciclava是一种潜在的PAO，不具有反硝化吸磷功能。除了 Propioniciclava 之外，Tessaracaccus 和 Thiothrix 也在两个系统中富集。总的来说，这项研究提出了一种新型的潜在 PAO，并拓宽了对 EBPR 微生物群落的理解。
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