MYB44 played key roles in plant responses to abiotic and biotic stress and important roles in plant metabolism and development. At present, the function of MYB44 has not been systemically summarized in plants. In this review, we systemically summarized the structure and function of MYB44 in plants, such as how MYB44 interplays in phytohormone signaling pathways; how MYB44 regulates abiotic and biotic stress, which includes drought tolerance, salt tolerance, cold tolerance, responding to phosphate and nitrogen starvation, and disease resistance; and how MYB44 regulates plant metabolism, which contains MYB44 regulates fruit malate accumulation, starch biosynthesis, sucrose accumulation, flavonoid accumulation, anthocyanin biosynthesis, chlorophyll degradation, and leaf senescence; it also regulates plant development, which includes root growth and development and somatic embryogenesis in plants. Moreover, we constructed the regulatory networks of the MYB44 protein's responses to biotic and abiotic stress and their regulation of plant metabolism and development. Furthermore, we give some suggestions on how to use MYB44 as a positive and negative regulator to create new breeds in the future.