<br>大型饮用水处理和分配系统中水细菌群落中假定致病菌种类的变化,Water Research

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大型饮用水处理和分配系统中水细菌群落中假定致病菌种类的变化
Water Research ( IF 11.4 ) Pub Date : 2023-11-30 , DOI: 10.1016/j.watres.2023.120947
Bei Zhao ₁ , Ruyin Liu ₂ , Yuxian Li ₁ , Hao Xu ₁ , Xiangyi Li ₁ , Junnong Gu ₁ , Xiaolan Zhang ₁ , Yue Wang ₃ , Yansong Wang ₄

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尽管饮用水中微生物的管理对于公共卫生至关重要，但在物种层面全面检查供水系统中的致病菌仍然存在挑战。本研究通过对近全长16S rRNA基因进行高通量测序，研究了三个大型饮用水处理厂（DWTP）及其相应分配系统在冬季和夏季的水细菌群落变化。我们的研究结果揭示了每个 DWTP 及其分配管理区域 (DMA) 的冬季和夏季水样之间的细菌群落结构存在显着差异。在地下水补给的 DWTP 中，两个季节都观察到分枝杆菌物种的选择性富集，随后的 DMA 也表现出对特定分枝杆菌物种的强烈选择。在其中一个地表水供水的分布式污水处理厂中，冬季源水中存在的某些军团菌在预氧化后选择性地富集在细菌群落中，尽管它们容易受到后续净化步骤的影响。根据我们的病原体识别管道，鉴定了多种假定的致病菌种 (n = 83)，其中优势种代表供水系统中常见的机会性病原体。虽然病原体去除主要发生在 DWTP 的净化过程中，特别是对于地表水喂养的植物，但 DMA 水体菌群中病原菌的相对丰度低于 DWTP 出水菌群中的相对丰度，表明 DMA 内病原体的竞争力下降生态系统。

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Changes of putative pathogenic species within the water bacterial community in large-scale drinking water treatment and distribution systems

Although the management of microbes in drinking water is of paramount importance for public health, there remain challenges in comprehensively examining pathogenic bacteria in the water supply system at the species level. In this study, high-throughput sequencing of nearly full-length 16S rRNA genes was performed to investigate the changes of the water bacterial community in three large-scale drinking water treatment plants (DWTPs) and their corresponding distribution systems during winter and summer. Our findings revealed significant differences in the bacterial community structure between winter and summer water samples for each DWTP and its distribution management area (DMA). In the groundwater-fed DWTP, selective enrichment of mycobacterial species was observed in both seasons, and the subsequent DMA also exhibited strong selection for specific mycobacterial species. In one of the surface water-fed DWTPs, certain Legionella species present in the source water in winter were selectively enriched in the bacterial community after pre-oxidation, although they were susceptible to the subsequent purification steps. A variety of putative pathogenic species (n = 83) were identified based on our pathogen identification pipeline, with the dominant species representing opportunistic pathogens commonly found in water supply systems. While pathogen removal primarily occurred during the purification processes of DWTPs, especially for surface water-fed plants, the relative abundance of pathogenic bacteria in the DMA water flora was lower than that in the DWTP effluent flora, indicating a diminished competitiveness of pathogens within the DMA ecosystem.

更新日期：2023-11-30

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