<br>两亲性嵌段共聚物 PEG-b-PMTC：合成、自组装、降解特性和生物相容性,Biomacromolecules

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两亲性嵌段共聚物 PEG-b-PMTC：合成、自组装、降解特性和生物相容性
Biomacromolecules ( IF 5.5 ) Pub Date : 2023-12-01 , DOI: 10.1021/acs.biomac.3c00992
Yiyi Deng _{1,

2} , Sven Schäfer ₃ , Devin Kronstein _{1,

2} , Azra Atabay ₁ , Moritz Susewind ₃ , Elisha Krieg _{1,

2} , Sebastian Seiffert ₃ , Jens Gaitzsch ₁

Affiliation

作为一种亲水性环状乙烯酮缩醛 (CKA)，2-亚甲基-1,3,6-三氧辛烷 (MTC) 最近引起了广泛关注，因为与其他经过深入研究的 CKA 相比，它能够促进更快的（生物）降解。。在这里，我们通过自由基开环聚合制备了基于具有不同链长的聚MTC的两亲性嵌段共聚物。这些两亲物在 PBS 缓冲液中进行自组装，生成尺寸为 40 至 105 nm 的纳米颗粒，并通过动态光散射、电子显微镜和静态光散射（Zimm 图）进行验证。随后，应用荧光光谱研究尼罗红负载纳米粒子的酶促降解。通过对纳米颗粒之间的荧光强度下降模式进行逐点比较，我们证明来自洋葱假单胞菌的脂肪酶在降解纳米颗粒方面非常有效。也在碱性条件下进行水解降解，4 h后实现完全降解。此外，对 HEK293 细胞进行了细胞毒性测定，结果证实，与高达 1 mg/mL 的纳米颗粒一起孵育 24 小时时，细胞活力超过 90%。因此，这些可生物降解和生物相容的纳米颗粒在药物释放等未来应用中具有巨大的潜力。

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Amphiphilic Block Copolymers PEG-b-PMTCs: Synthesis, Self-Assembly, Degradation Properties and Biocompatibility

As a hydrophilic cyclic ketene acetal (CKA), 2-methylene-1,3,6-trioxocane (MTC) has recently attracted a lot of attention owing to its ability to promote a quicker (bio)degradation as compared to other heavily studied CKAs. Here, we prepared amphiphilic block copolymers based on poly-MTC with varying chain lengths by radical ring opening polymerization. Self-assemblies of these amphiphiles were performed in PBS buffer to generate nanoparticles with sizes from 40 to 105 nm, which were verified by dynamic light scattering, electron microscopy, and static light scattering (Zimm plots). Subsequently, fluorescence spectroscopy was applied to study the enzymatic degradation of Nile red-loaded nanoparticles. By performing a point-by-point comparison of fluorescence intensity decline patterns between nanoparticles, we demonstrated that lipase from Pseudomonas cepacia was very efficient in degrading the nanoparticles. Hydrolysis degradations under basic conditions were also carried out, and a complete degradation was achieved after 4 h. Additionally, cytotoxicity assays were carried out on HEK293 cells, and the results affirmed cell viabilities over 90% when incubated with up to 1 mg/mL nanoparticles for 24 h. These biodegradable and biocompatible nanoparticles hence hold great potential for future applications such as drug release.

更新日期：2023-12-01

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