<br>研究超极化 [1-13C] 丙酮酸注射后麻醉健康大鼠脑体内 13C 乳酸信号的来源,NMR in Biomedicine

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研究超极化 [1-13C] 丙酮酸注射后麻醉健康大鼠脑体内 13C 乳酸信号的来源
NMR in Biomedicine ( IF 2.7 ) Pub Date : 2023-11-22 , DOI: 10.1002/nbm.5073
Minjie Zhu ₁ , Aditya Jhajharia ₁ , Sonal Josan ₂ , Jae Mo Park _{3,

4,

5} , Yi-Fen Yen ₆ , Adolf Pfefferbaum ₇ , Ralph E Hurd ₈ , Daniel M Spielman ₈ , Dirk Mayer _{1,

9}

Affiliation

本研究的目的是调查健康麻醉大鼠注射超极化（HP） [^1-13C] 丙酮酸（Pyr）后脑乳酸（Lac）信号的来源。具有血流敏化梯度的动态二维螺旋化学位移成像显示血管和脑 Pyr 均降低，而 Lac 对血流抑制水平没有显着依赖性。这些结果支持 HP 代谢物主要存在于大脑的不同区室（即血液中的 Pyr 和薄壁组织中的 Lac）的假设。来自 [^1-13C] Pyr 的高分辨率代谢成像数据进一步表明，在大脑中检测到的 Lac 并非来自部分体积效应引起的血管信号的贡献。此外，注射 HP [^1-13C]Lac 后用动态成像测量的代谢物分布和动力学与使用 Pyr 作为底物时的 Pyr 数据相似。这些数据不支持以下假设：Pyr 注射后在大脑中观察到的 Lac 在其他器官中产生，然后穿过血脑屏障（BBB）运输。总之，所提出的结果提供了进一步的证据，即使在健康的麻醉大鼠中，HP Pyr 在 BBB 上的运输也足够快，可以检测到其在大脑内代谢转化为 Lac。

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Investigating the origin of the 13C lactate signal in the anesthetized healthy rat brain in vivo after hyperpolarized [1-13C]pyruvate injection

The goal of this study was to investigate the origin of brain lactate (Lac) signal in the healthy anesthetized rat after injection of hyperpolarized (HP) [1-¹³C]pyruvate (Pyr). Dynamic two-dimensional spiral chemical shift imaging with flow-sensitizing gradients revealed reduction in both vascular and brain Pyr, while no significant dependence on the level of flow suppression was detected for Lac. These results support the hypothesis that the HP metabolites predominantly reside in different compartments in the brain (i.e., Pyr in the blood and Lac in the parenchyma). Data from high-resolution metabolic imaging of [1-¹³C]Pyr further demonstrated that Lac detected in the brain was not from contributions of vascular signal attributable to partial volume effects. Additionally, metabolite distributions and kinetics measured with dynamic imaging after injection of HP [1-¹³C]Lac were similar to Pyr data when Pyr was used as the substrate. These data do not support the hypothesis that Lac observed in the brain after Pyr injection was generated in other organs and then transported across the blood–brain barrier (BBB). Together, the presented results provide further evidence that even in healthy anesthetized rats, the transport of HP Pyr across the BBB is sufficiently fast to permit detection of its metabolic conversion to Lac within the brain.

更新日期：2023-11-22

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