Human induced pluripotent stem cells (iPSCs) hold great promise for personalized medicine, as they can be differentiated into specific cell types, especially mesenchymal stem cells (MSCs). Therefore, our study sought to assess the feasibility of deriving MSCs from teratomas generated from human iPSCs. Teratomas serve as a model to mimic multilineage human development, thus enriching specific somatic progenitors and stem cells. Here, we discovered a small, condensed mass of MSCs within iPSC-generated teratomas. Afterward, we successfully isolated MSCs from this condensed mass, which was a byproduct of teratoma development. To evaluate the characteristics and cell behaviors of iPSC-derived MSCs (iPSC-MSCs), we conducted comprehensive assessments using qPCR, immunophenotype analysis, and cell proliferation-related assays. Remarkably, iPSC-MSCs exhibited an immunophenotype resembling that of conventional MSCs, and they displayed robust proliferative capabilities, similar to those of higher pluripotent stem cell-derived MSCs. Furthermore, iPSC-MSCs demonstrated the ability to differentiate into multiple lineages in vitro. Finally, we evaluated the therapeutic potential of iPSC-MSCs using an osteochondral defect model. Our findings demonstrated that teratomas are a promising source for the isolation of condensed MSCs. More importantly, our results suggest that iPSC-MSCs derived from teratomas possess the capacity for tissue regeneration, highlighting their promise for future therapeutic applications.

中文翻译：

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人 iPSC 衍生畸胎瘤间充质干细胞对骨软骨缺损再生的治疗潜力

人类诱导多能干细胞（iPSC）在个性化医疗方面具有广阔的前景，因为它们可以分化为特定的细胞类型，尤其是间充质干细胞（MSC）。因此，我们的研究试图评估从人类 iPSC 产生的畸胎瘤中衍生 MSC 的可行性。畸胎瘤作为模仿人类多谱系发育的模型，从而丰富了特定的体细胞祖细胞和干细胞。在这里，我们在 iPSC 生成的畸胎瘤中发现了一小块浓缩的 MSC。随后，我们成功地从这团凝结物中分离出了间充质干细胞，这是畸胎瘤发育的副产品。为了评估 iPSC 衍生的 MSC (iPSC-MSC) 的特征和细胞行为，我们使用 qPCR、免疫表型分析和细胞增殖相关测定进行了全面评估。值得注意的是，iPSC-MSC 表现出与传统 MSC 相似的免疫表型，并且它们表现出强大的增殖能力，与高级多能干细胞衍生的 MSC 相似。此外，iPSC-MSC 表现出体外分化为多个谱系的能力。最后，我们使用骨软骨缺损模型评估了 iPSC-MSC 的治疗潜力。我们的研究结果表明，畸胎瘤是分离浓缩间充质干细胞的一个有前途的来源。更重要的是，我们的结果表明，源自畸胎瘤的 iPSC-MSC 具有组织再生的能力，凸显了它们在未来治疗应用中的前景。