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Smart Ratiometric SERS Nanoprobe for Real-Time Monitoring Hydrogen Peroxide in Living Cells during NADH Treatment Associated with Ferroptosis
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-11-29 , DOI: 10.1021/acs.analchem.3c02912 Dan Sun 1 , Guohua Qi 2 , Xuan Yi 1 , Hongyan Zhu 1 , Yongdong Jin 2, 3
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-11-29 , DOI: 10.1021/acs.analchem.3c02912 Dan Sun 1 , Guohua Qi 2 , Xuan Yi 1 , Hongyan Zhu 1 , Yongdong Jin 2, 3
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Studying the oxidative stress, especially the reactive oxygen species (ROS) response of ferroptosis, is crucial for the diagnosis and treatment of cancer based on ferroptosis. However, reliable quantitative analysis of intracellular ROS in cancer treatment for drug screening is still a challenge. Herein, a superior ratiometric SERS nanoprobe was developed for in situ, real-time, and highly sensitive detection of content variation of H2O2 within living cells. The SERS nanoprobe was prepared by coassembly of the internal standard molecule p-mercaptobenzonitrile and the reporter molecule p-mercaptophenylboronic acid on the surface of gold nanoparticles, used for synergistic calibration and detection of H2O2, which enables reliable detection of the true content of intracellular H2O2 without the interference of other substances in cells. Based on the nanoprobe, we found that the level of intracellular H2O2 of cancer cells was increased after the nicotinamide adenine dinucleotide (NADH) treatment, with a dose-dependence to the concentration of NADH. High doses of NADH (above 20 mM) can induce cell death by means of ferroptosis associated with the level elevation of intracellular lipid hydroperoxides. This study highlights the potential of the SERS nanoprobe for tracking content variation of cellular H2O2 and understanding its roles in screening new anticancer drugs.
中文翻译：
智能比例 SERS 纳米探针，用于在与铁死亡相关的 NADH 治疗期间实时监测活细胞中的过氧化氢
研究铁死亡的氧化应激，特别是活性氧（ROS）反应，对于基于铁死亡的癌症的诊断和治疗至关重要。然而，在癌症治疗中对细胞内ROS进行可靠的定量分析以进行药物筛选仍然是一个挑战。在此，开发了一种卓越的比例SERS纳米探针，用于原位、实时、高灵敏度地检测活细胞内H 2 O 2的含量变化。 SERS纳米探针是将内标分子对巯基苯甲腈和报告分子对巯基苯硼酸共组装在金纳米粒子表面，用于协同校准和检测H 2 O 2 ，能够可靠地检测真实含量细胞内H 2 O 2的产生，不受细胞内其他物质的干扰。基于纳米探针，我们发现癌细胞的细胞内H 2 O 2水平在烟酰胺腺嘌呤二核苷酸（NADH）处理后增加，且与NADH的浓度呈剂量依赖性。高剂量的 NADH（高于 20 mM）可通过与细胞内脂质氢过氧化物水平升高相关的铁死亡来诱导细胞死亡。这项研究强调了 SERS 纳米探针在跟踪细胞 H 2 O 2含量变化并了解其在筛选新抗癌药物中的作用的潜力。
更新日期：2023-11-29
中文翻译：
智能比例 SERS 纳米探针，用于在与铁死亡相关的 NADH 治疗期间实时监测活细胞中的过氧化氢
研究铁死亡的氧化应激，特别是活性氧（ROS）反应，对于基于铁死亡的癌症的诊断和治疗至关重要。然而，在癌症治疗中对细胞内ROS进行可靠的定量分析以进行药物筛选仍然是一个挑战。在此，开发了一种卓越的比例SERS纳米探针，用于原位、实时、高灵敏度地检测活细胞内H 2 O 2的含量变化。 SERS纳米探针是将内标分子对巯基苯甲腈和报告分子对巯基苯硼酸共组装在金纳米粒子表面，用于协同校准和检测H 2 O 2 ，能够可靠地检测真实含量细胞内H 2 O 2的产生，不受细胞内其他物质的干扰。基于纳米探针，我们发现癌细胞的细胞内H 2 O 2水平在烟酰胺腺嘌呤二核苷酸（NADH）处理后增加，且与NADH的浓度呈剂量依赖性。高剂量的 NADH（高于 20 mM）可通过与细胞内脂质氢过氧化物水平升高相关的铁死亡来诱导细胞死亡。这项研究强调了 SERS 纳米探针在跟踪细胞 H 2 O 2含量变化并了解其在筛选新抗癌药物中的作用的潜力。
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