The design and construction of high-performance protein absorbents are of great significance for obtaining highly purified proteins in the field of biotechnology and more. In this study, the highly carboxylated absorption media were fabricated for selective absorption of positively charged proteins by using bacterial cellulose (BC) nanofibrous aerogel (NFA) as a substrate material in concert with citric acid (CA). The obtained BC/CA NFAs exhibited highly interconnected open porous structure, surface hydrophilicity, large specific surface area (14.2 m²/g), good elasticity and compression fatigue resistance. BC/CA NFAs displayed a high lysozyme absorption capacity of 868.9 mg/g and fast equilibrium within 2.5 h. A dynamic lysozyme absorption capacity of 655.35 mg/g was achieved under the drive of gravity, meeting the demands of actual applications. Furthermore, BC/CA NFAs exhibited unique absorption selectivity performance, good reusability, as well as acid and alkaline resistance. A successful scale-up of such environmental friendliness, low cost and good reproducibility absorbents could provide a new perspective to develop next generation three-dimensional chromatographic media for substantial bio-separation applications.

Graphical abstract

中文翻译：

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接枝柠檬酸的细菌纤维素纳米纤维气凝胶用于蛋白质的吸收和分离

高性能蛋白质吸附剂的设计和构建对于生物技术等领域获得高纯度蛋白质具有重要意义。在这项研究中，通过使用细菌纤维素（BC）纳米纤维气凝胶（NFA）作为基质材料与柠檬酸（CA）配合，制造了高度羧化的吸收介质，用于选择性吸收带正电的蛋白质。所得BC/CA NFA表现出高度互连的开孔结构、表面亲水性、大的比表面积(14.2 m ² /g)、良好的弹性和抗压缩疲劳性。BC/CA NFA 表现出 868.9 mg/g 的高溶菌酶吸收能力，并在 2.5 小时内快速达到平衡。在重力驱动下，溶菌酶动态吸附量达到655.35 mg/g，满足实际应用需求。此外，BC/CA NFA还表现出独特的吸收选择性性能、良好的可重复使用性以及耐酸碱性。这种环境友好、低成本和良好重现性的吸收剂的成功放大可以为开发用于大量生物分离应用的下一代三维色谱介质提供新的视角。

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