<br>不溶性/可溶性分数比决定膳食纤维对健康小鼠肠道微生物群和血清代谢物的影响,Food & Function

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不溶性/可溶性分数比决定膳食纤维对健康小鼠肠道微生物群和血清代谢物的影响
Food & Function ( IF 5.1 ) Pub Date : 2023-11-28 , DOI: 10.1039/d3fo04068b
Yanli Zhang ₁ , Jielun Hu ₁ , Yadong Zhong ₁ , Shuai Liu ₁ , Liandi Liu ₁ , Xinyi Mu ₁ , Chunhua Chen ₁ , Shenji Yang ₁ , Guohao Li ₁ , Duoduo Zhang ₁ , Xinru Huang ₁ , Jinrui Yang ₁ , Xiaojun Huang ₁ , Shuigen Bian ₁ , Shaoping Nie ₁

Affiliation

可溶性膳食纤维（SDF）和不溶性膳食纤维（IDF）在维持肠道菌群稳态中发挥着关键作用； IDF 和 SDF 不同比例的效果是否一致仍不清楚。因此，我们从六种代表性食物（苹果、芹菜、羽衣甘蓝、黑木耳、燕麦和大豆）中筛选出SDF和IDF，并配制了IDF和SDF比例为1:9至9:1的9种膳食纤维配方（NDFR ）将它们对微生物的影响与健康小鼠进行比较。我们发现 NDFR 处理降低了门水平上变形菌门的丰度和厚壁菌门/拟杆菌门的比例。随着IDF比例的增加，属水平的Parabacteroides和Prevotella的α多样性和相对丰富度呈上升趋势，而Akkermansia在属水平的相对丰度以及乙酸和丙酸的产量则随着IDF比例的增加呈上升趋势。随着SDF比例的增加。 I9S1DF组（IDF与SDF比例为9:1）中副杆菌属和普氏菌相对丰度分别是I1S9DF组（IDF与SDF比例为1:9）的1.72倍和5.92倍。 I1S9DF组中Akkermansia的相对丰度是I9S1DF组的17.18倍。而且高比例的SDF（SDF达到60%以上）丰富了甘油磷脂代谢途径；然而，高比例的IDF（IDF达到80％或更高）调节三羧酸循环。这些发现有助于开发基于肠道微生物群和代谢物的膳食纤维补充剂。

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Insoluble/soluble fraction ratio determines effects of dietary fiber on gut microbiota and serum metabolites in healthy mice

Both soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) play pivotal roles in maintaining gut microbiota homeostasis; whether the effects of the different ratios of IDF and SDF are consistent remains unclear. Consequently, we selected SDFs and IDFs from six representative foods (apple, celery, kale, black fungus, oats, and soybeans) and formulated nine dietary fiber recipes composed of IDF and SDF with a ratio from 1 : 9 to 9 : 1 (NDFR) to compare their impact on microbial effects with healthy mice. We discovered that NDFR treatment decreased the abundance of Proteobacteria and the ratio of Firmicutes/Bacteroidetes at the phylum level. The α diversity and relative richness of Parabacteroides and Prevotella at the genus level showed an upward trend along with the ratio of IDF increasing, while the relative abundance of Akkermansia at the genus level and the production of acetic acid and propionic acid exhibited an increased trend along with the ratio of SDF increasing. The relative abundance of Parabacteroides and Prevotella in the I9S1DF group (the ratio of IDF and SDF was 9 : 1) was 1.72 times and 5.92 times higher than that in the I1S9DF group (the ratio of IDF and SDF was 1 : 9), respectively. The relative abundance of Akkermansia in the I1S9DF group was 17.18 times higher than that in the I9S1DF group. Moreover, a high ratio of SDF (SDF reaches 60% or more) enriched the glycerophospholipid metabolism pathway; however, a high ratio of IDF (IDF reaches 80% or more) regulated the tricarboxylic acid cycle. These findings are helpful in the development of dietary fiber supplements based on gut microbiota and metabolites.

更新日期：2023-11-28

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