Microalgal species from sewage treatment plant were identified by 18S rRNA sequencing and were explored for total lipids, carbohydrate, and protein contents, to serve as a potential candidate for biorefinery. Seven unicellular microalgae were identified as Chlorella sorokiniana, Dictyosphaerium sp., Graesiella emersonii belonging to Chlorellaceae and Scenedesmus sp., Desmodesmus sp., Tetranephris brasiliensis, and Coelastrella sp. belonging to Scenedesmaceae family. Biochemical assessment of all isolates revealed total lipid content from 17.49 ± 1.41 to 47.35 ± 0.61% w/w, total carbohydrate content from 12.82 ± 0.19 to 64.29 ± 0.63% w/w, and total protein content from 8.55 ± 0.19 to 16.65 ± 0.20% w/w. FAME analysis of extracted lipid was found to be rich in Hexadecane (C16:0), Tetradecane (C17:0), Octadecane (C18:0), Eicosane (C20:0), Tetracosane (C24:0), Pentacosane (C25:0) fatty acids, the presence of which makes excellent candidate for biodiesel. Being rich in lipid, microalgae Chlorella sorokiniana, Coelastrella sp., and Scenedesmus sp. have high potential for biofuels. Due to the presence of high protein content, Scenedesmus sp. and Chlorella sorokiniana can serve as food or feed supplement, whereas the high carbohydrate content of Dictyosphaerium sp., Coelastrella sp., and Scenedesmus sp. makes them an ideal candidate for fermentative production of alcohol and organic acids. Chlorella sp. and Scenedesmus sp., being dominant microalgae across all seasons, demonstrate remarkable resilience for their cultivation in sewage water and utilization of biomass in biorefineries.

通过 18S rRNA 测序鉴定来自污水处理厂的微藻物种，并探索其总脂质、碳水化合物和蛋白质含量，作为生物精炼的潜在候选者。7种单细胞微藻被鉴定为Chlorella sorokiniana、Dictyosphaerium sp.、Graesiella emersonii ，属于小球藻科和栅藻属sp.、Desmodesmus sp.、Tetranephris brasiliensis和Coelastrella sp.。属于栅藻科。所有分离株的生化评估显示总脂质含量为 17.49 ± 1.41 至 47.35 ± 0.61% w/w，总碳水化合物含量为 12.82 ± 0.19 至 64.29 ± 0.63% w/w，总蛋白质含量为 8.55 ± 0.19 至 16.65 ± 0.20 % w/w。提取脂质的 FAME 分析发现富含十六烷 (C16:0)、十四烷 (C17:0)、十八烷 (C18:0)、二十烷 (C20:0)、二十四烷 (C24:0)、二十五烷 (C25: 0) 脂肪酸，它的存在使其成为生物柴油的绝佳候选者。微藻Chlorella sorokiniana、Coelastrella sp. 和Scenedesmus sp.富含脂质。具有开发生物燃料的巨大潜力。由于存在高蛋白质含量，栅藻sp。和Chlorella sorokiniana可以作为食品或饲料补充剂，而Dictyosphaerium sp.、Coelastrella sp. 和Scenedesmus sp. 的碳水化合物含量较高。使它们成为酒精和有机酸发酵生产的理想选择。小球藻属 和栅藻是所有季节的主要微藻，它们在污水中的培养和生物精炼厂中生物质的利用方面表现出卓越的适应能力。