In this research work, a magnetic nanobiocomposite is designed and presented based on the extraction of flaxseed mucilage hydrogel, silk fibroin (SF), and Fe₃O₄ magnetic nanoparticles (Fe₃O₄ MNPs). The physiochemical features of magnetic flaxseed mucilage hydrogel/SF nanobiocomposite are evaluated by FT-IR, EDX, FE-SEM, TEM, XRD, VSM, and TG technical analyses. In addition to chemical characterization, given its natural-based composition, the in-vitro cytotoxicity and hemolysis assays are studied and the results are considerable. Following the use of highest concentration of magnetic flaxseed mucilage hydrogel/SF nanobiocomposite (1.75 mg/mL) and the cell viability percentage of two different cell lines including normal HEK293T cells (95.73%, 96.19%) and breast cancer BT549 cells (87.32%, 86.9%) in 2 and 3 days, it can be inferred that this magnetic nanobiocomposite is biocompatible with HEK293T cells and can inhibit the growth of BT549 cell lines. Besides, observing less than 5% of hemolytic effect can confirm its hemocompatibility. Furthermore, the high specific absorption rate value (107.8 W/g) at 200 kHz is generated by a determined concentration of this nanobiocomposite (1 mg/mL). According to these biological assays, this magnetic responsive cytocompatible composite can be contemplated as a high-potent substrate for further biomedical applications like magnetic hyperthermia treatment and tissue engineering.

在这项研究工作中，基于亚麻籽粘液水凝胶、丝素蛋白（SF）和Fe ₃ O ₄磁性纳米粒子（Fe ₃ O ₄ MNP）的提取，设计并提出了一种磁性纳米生物复合材料。通过 FT-IR、EDX、FE-SEM、TEM、XRD、VSM 和 TG 技术分析评估磁性亚麻籽粘液水凝胶/SF 纳米生物复合材料的理化特征。除了化学表征之外，考虑到其天然成分，还研究了体外细胞毒性和溶血测定，结果相当可观。使用最高浓度的磁性亚麻籽粘液水凝胶/SF纳米生物复合材料（1.75 mg/mL）和两种不同细胞系的细胞活力百分比，包括正常HEK293T细胞（95.73%、96.19%）和乳腺癌BT549细胞（87.32%） 86.9%）在2和3天内，可以推断这种磁性纳米生物复合材料与HEK293T细胞具有生物相容性，并且可以抑制BT549细胞系的生长。此外，观察到小于5%的溶血效应即可证实其血液相容性。此外，200 kHz 下的高比吸收率值 (107.8 W/g) 是由该纳米生物复合材料的确定浓度 (1 mg/mL) 产生的。根据这些生物测定，这种磁响应细胞相容性复合材料可以被视为一种高效基质，用于进一步的生物医学应用，如磁热疗和组织工程。