Function and Structure of a Terpene Synthase Encoded in a Giant Virus Genome,Journal of the American Chemical Society

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Function and Structure of a Terpene Synthase Encoded in a Giant Virus Genome
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2023-11-27 , DOI: 10.1021/jacs.3c10603
Youngcheol Jung ₁ , Takaaki Mitsuhashi ₂ , Sota Sato _{1,

2} , Miki Senda ₃ , Toshiya Senda ₃ , Makoto Fujita _{1,

2,

4}

Affiliation

Giant viruses are nonstandard viruses with large particles and genomes. While previous studies have shown that their genomes contain various sequences of interest, their genes related specifically to natural product biosynthesis remain unexplored. Here we analyze the function and structure of a terpene synthase encoded by the gene of a giant virus. The enzyme is phylogenetically separated from the terpene synthases of cellular organisms; however, heterologous gene expression revealed that it still functions as a terpene synthase and produces a cyclic terpene from a farnesyl diphosphate precursor. Crystallographic analysis revealed its protein structure, which is relatively compact but retains essential motifs of the terpene synthases. We thus suggest that like cellular organisms, giant viruses produce and utilize natural products for their ecological strategies.

中文翻译：

巨型病毒基因组中编码的萜烯合酶的功能和结构

巨型病毒是具有大颗粒和基因组的非标准病毒。虽然之前的研究表明它们的基因组包含各种感兴趣的序列，但它们与天然产物生物合成具体相关的基因仍未被探索。在这里，我们分析了巨型病毒基因编码的萜烯合酶的功能和结构。该酶在系统发育上与细胞生物的萜烯合酶分开；然而，异源基因表达表明它仍然发挥萜烯合酶的作用，并从法尼基二磷酸前体产生环状萜烯。晶体分析揭示了其蛋白质结构，该结构相对紧凑，但保留了萜烯合酶的基本基序。因此，我们认为，像细胞生物一样，巨型病毒也生产并利用天然产物来实现其生态策略。

更新日期：2023-11-27

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