<br>抑制皮肤热敏 Ca2+ 可渗透瞬时受体电位香草酸 3 通道可减轻 UVB 诱导的小鼠皮肤损伤,The FASEB Journal

当前位置： X-MOL 学术 › FASEB J. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

抑制皮肤热敏 Ca2+ 可渗透瞬时受体电位香草酸 3 通道可减轻 UVB 诱导的小鼠皮肤损伤
The FASEB Journal ( IF 4.4 ) Pub Date : 2023-11-20 , DOI: 10.1096/fj.202301591rr
Yaxuan Qu ₁ , Xiaoying Sun _{1,

2} , Ningning Wei _{1,

2} , KeWei Wang _{1,

2}

Affiliation

紫外线 B (UVB) 辐射会引发皮肤角质形成细胞中的过量钙流入和信号级联反应，从而导致皮肤损伤。在角质形成细胞中强烈表达的热敏性Ca ²⁺渗透性瞬时受体电位香草酸3 (TRPV3) 通道在皮肤屏障形成和伤口愈合中发挥重要作用。在此，我们报告抑制皮肤 TRPV3 可减轻 UVB 辐射引起的皮肤损伤。在单次暴露于弱 UVB 辐射引起的耳朵肿胀和背部皮肤损伤的小鼠模型中，定量实时 PCR 和蛋白质印迹测定中 TRPV3 基因和蛋白质上调。随着TRPV3的上调，促炎细胞因子肿瘤坏死因子-α（TNF-α）和白介素-6（IL-6）的表达也增加。 TRPV3基因的敲除可减轻 UVB 引起的耳朵肿胀和背部皮肤炎症。此外，两种选择性 TRPV3 抑制剂蛇床子素和毛蕊花苷的局部应用导致皮肤炎症和病变的剂量依赖性减弱。总而言之，我们的研究结果证明了过度活跃的 TRPV3 通道功能在 UVB 引起的皮肤损伤的发生中的致病作用。因此，局部抑制 TRPV3 可能对 UVB 辐射引起的皮肤损伤具有潜在的治疗或预防作用。

"点击查看英文标题和摘要"

Inhibition of cutaneous heat-sensitive Ca2+-permeable transient receptor potential vanilloid 3 channels alleviates UVB-induced skin lesions in mice

Ultraviolet B (UVB) radiation causes skin injury by trigging excessive calcium influx and signaling cascades in the skin keratinocytes. The heat-sensitive Ca²⁺-permeable transient receptor potential vanilloid 3 (TRPV3) channels robustly expressed in the keratinocytes play an important role in skin barrier formation and wound healing. Here, we report that inhibition of cutaneous TRPV3 alleviates UVB radiation-induced skin lesions. In mouse models of ear swelling and dorsal skin injury induced by a single exposure of weak UVB radiation, TRPV3 genes and proteins were upregulated in quantitative real-time PCR and Western blot assays. In accompany with TRPV3 upregulations, the expressions of proinflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were also increased. Knockout of the TRPV3 gene alleviates UVB-induced ear swelling and dorsal skin inflammation. Furthermore, topical applications of two selective TRPV3 inhibitors, osthole and verbascoside, resulted in a dose-dependent attenuation of skin inflammation and lesions. Taken together, our findings demonstrate the causative role of overactive TRPV3 channel function in the development of UVB-induced skin injury. Therefore, topical inhibition of TRPV3 may hold potential therapy or prevention of UVB radiation-induced skin injury.

更新日期：2023-11-20

点击分享查看原文

点击收藏

阅读更多本刊新发论文