<br>掺有角蛋白和 VEGF 的蜂蜜海绵纳米纤维敷料：伤口愈合的理想结构,ACS Applied Materials & Interfaces

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掺有角蛋白和 VEGF 的蜂蜜海绵纳米纤维敷料：伤口愈合的理想结构
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2023-11-21 , DOI: 10.1021/acsami.3c11093
Mohamadreza Tavakoli _{1,

2} , Marjan Mirhaj _{1,

2} , Jaleh Varshosaz ₃ , Mastafa H Al-Musawi ₄ , Yasir Q Almajidi ₅ , Amir Mohammad Danesh Pajooh ₆ , Mina Shahriari-Khalaji ₇ , Fariborz Sharifianjazi ₈ , Mansoor Alizadeh ₉ , Sheyda Labbaf ₂ , Kimia Eslami Shahrebabaki ₂ , Pegah Madani Nasab ₂ , Mahboubeh Firuzeh ₂ , Salar Nasr Esfahani ₁₀

Affiliation

Pharmacy Student's Research Committee, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran.
Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Novel Drug Delivery Systems Research Centre, Department of Pharmaceutics, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran.
Department of Clinical Laboratory Science, College of Pharmacy, Mustansiriyah University, Baghdad 10052, Iraq.
Department of Pharmacy (Pharmaceutics), Baghdad College of Medical Sciences, Baghdad 10047, Iraq.
Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran 1439956191, Iran.
State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China.
Department of Natural Sciences, School of Science and Technology, University of Georgia, Tbilisi 0171, Georgia.
Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran 1477893855, Iran.
Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan 8174673461, Iran.

为了克服单层伤口敷料的缺点，现在引入双层敷料作为替代方案，以实现有效和长期的治疗。这里，提出了一种双层敷料，由底层（BL）中的电纺纳米纤维和顶层（TL）中的海绵结构组成。制备具有76.04μm互连孔的亲水性聚丙烯酸（PAAc）-蜂蜜（Hny）作为TL，并制备角蛋白（Kr）、Hny和血管内皮生长因子（VEGF）作为BL。鸡绒毛尿囊膜测定和体内组织形态学观察证明，VEGF 在 7 天内逐渐释放，促进血管生成。此外，制造的敷料材料显示出令人满意的拉伸曲线、人类角质形成细胞的细胞相容性以及促进细胞附着和迁移的能力。体内动物模型表明，当用 PAAc-Hny/Hny-Kr-VEGF 覆盖时，全层伤口愈合速度比其他组更快。此外，还证实了更快的血管形成、胶原蛋白合成和表皮层生成，这证明使用合成双层敷料治疗的伤口可以有效加速愈合。我们的研究结果表明，所制造的材料有望作为功能性伤口敷料。

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Keratin- and VEGF-Incorporated Honey-Based Sponge–Nanofiber Dressing: An Ideal Construct for Wound Healing

To overcome the drawbacks of single-layered wound dressings, bilayer dressings are now introduced as an alternative to achieve effective and long-term treatment. Here, a bilayer dressing composed of electrospun nanofibers in the bottom layer (BL) and a sponge structure as the top layer (TL) is presented. Hydrophilic poly(acrylic acid) (PAAc)–honey (Hny) with interconnected pores of 76.04 μm was prepared as the TL and keratin (Kr), Hny, and vascular endothelial growth factor (VEGF) were prepared as the BL. VEGF indicates a gradual release over 7 days, promoting angiogenesis, as proven by the chick chorioallantoic membrane assay and in vivo tissue histomorphology observation. Additionally, the fabricated dressing material indicated a satisfactory tensile profile, cytocompatibility for human keratinocyte cells, and the ability to promote cell attachment and migration. The in vivo animal model demonstrated that the full-thickness wound healed faster when it was covered with PAAc–Hny/Hny–Kr–VEGF than in other groups. Additionally, faster blood vessel formation, collagen synthetization, and epidermal layer generation were also confirmed, which have proven efficient healing acceleration in wounds treated with synthesized bilayer dressings. Our findings indicated that the fabricated material can be promising as a functional wound dressing.

更新日期：2023-11-21

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