DNA tiles serve as the fundamental building blocks for DNA self-assembled nanostructures such as DNA arrays, origami, and designer crystals. Introducing additional binding arms to DNA crossover tiles holds the promise of unlocking diverse nano-assemblies and potential applications. Here, we present one-, two-, and three-layer T-shaped crossover tiles, by integrating T junction with antiparallel crossover tiles. These tiles carry over the orthogonal binding directions from T junction and retain the rigidity from antiparallel crossover tiles, enabling the assembly of various 2D tessellations. To demonstrate the versatility of the design rules, we create 2-state reconfigurable nanorings from both single-stranded tiles and single-unit assemblies. Moreover, four sets of 4-state reconfiguration systems are constructed, showing effective transformations between ladders and/or rings with pore sizes spanning ~20 nm to ~168 nm. These DNA tiles enrich the design tools in nucleic acid nanotechnology, offering exciting opportunities for the creation of artificial dynamic DNA nanopores.

DNA 瓦片是 DNA 自组装纳米结构（例如 DNA 阵列、折纸和设计晶体）的基本构建块。在 DNA 交叉瓷砖中引入额外的结合臂有望解锁不同的纳米组件和潜在应用。在这里，我们通过将 T 形连接与反平行交叉瓷砖相结合，展示了一层、两层和三层 T 形交叉瓷砖。这些瓦片继承了 T 形连接处的正交绑定方向，并保留了反平行交叉瓦片的刚性，从而能够组装各种 2D 镶嵌图案。为了证明设计规则的多功能性，我们从单链瓦片和单单元组件创建了二态可重构纳米环。此外，构建了四套 4 态重构系统，显示了孔径范围从约 20 nm 到约 168 nm 的梯子和/或环之间的有效转换。这些 DNA 瓦片丰富了核酸纳米技术的设计工具，为人工动态 DNA 纳米孔的创建提供了令人兴奋的机会。