<br>Indole-3-Lactic Acid（植物乳杆菌 DPUL-S164 的一种色氨酸代谢物）通过激活 AhR 和 Nrf2 信号通路改善肠道屏障损伤,Journal of Agricultural and Food Chemistry

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Indole-3-Lactic Acid（植物乳杆菌 DPUL-S164 的一种色氨酸代谢物）通过激活 AhR 和 Nrf2 信号通路改善肠道屏障损伤
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-11-23 , DOI: 10.1021/acs.jafc.3c06183
Arong Wang _{1,

2} , Cheng Guan _{1,

2} , Tieqi Wang _{1,

2} , Guangqing Mu _{1,

2} , Yanfeng Tuo _{1,

2}

Affiliation

越来越多的证据表明，微生物色氨酸代谢物在维持肠道屏障稳定性和调节宿主免疫力方面发挥着至关重要的作用。我们之前的研究表明，植物乳杆菌( L. plantarum ) DPUL-S164 对高色氨酸 (Trp) 饮食的小鼠进行干预，可促进小鼠肠道中吲哚-3-乳酸 (ILA) 的产生，并改善右旋糖酐硫酸钠 (DSS) ）引起的小鼠肠道屏障损伤。在本研究中，我们使用 HT-29 细胞单层模型来评估植物乳杆菌DPUL-S164 Trp 代谢物 (DPUL-S164-TM) 对肠道屏障的影响。我们发现植物乳杆菌DPUL-S164-TM 通过促进紧密连接蛋白（ZO-1、occludin、claudin1）的表达、激活AhR 和 Nrf2 信号通路，并抑制 NF-κB 信号通路。我们发现植物乳杆菌DPUL-S164-TM促进紧密连接蛋白表达和激活Nrf2信号通路依赖于HT-29细胞的AhR表达。此外，植物乳杆菌DPUL-S164-TM 显示 ILA 含量显着增加。因此，我们推测植物乳杆菌DPUL-S164-TM中的ILA在改善肠道屏障功能、减轻炎症方面发挥着关键作用。

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Indole-3-Lactic Acid, a Tryptophan Metabolite of Lactiplantibacillus plantarum DPUL-S164, Improved Intestinal Barrier Damage by Activating AhR and Nrf2 Signaling Pathways

A growing body of evidence suggests that microbial tryptophan metabolites play a crucial role in maintaining intestinal barrier stability and modulating host immunity. Our previous study showed that the Lactiplantibacillus plantarum (L. plantarum ) DPUL-S164 intervention in mice with a high tryptophan (Trp) diet promotes indole-3-lactic acid (ILA) production in the mice’s intestinal tract and ameliorates dextran sodium sulfate(DSS)-induced intestinal barrier damage in mice. In this study, we used the HT-29 cell monolayer model to evaluate the effect of the L. plantarum DPUL-S164 Trp metabolites (DPUL-S164-TM) on the intestinal barrier. We found that L. plantarum DPUL-S164-TM alleviated lipopolysaccharide (LPS)-induced intestinal barrier damage and inflammation of the HT-29 cell monolayer by promoting the expression of tight junction proteins (ZO-1, occludin, claudin1), activating the AhR and Nrf2 signaling pathways, and inhibiting the NF-κB signaling pathway. We found that the promotion of tight junction protein expression and the activation of the Nrf2 signaling pathway by L. plantarum DPUL-S164-TM were dependent on the AhR expression of HT-29 cells. Additionally, L. plantarum DPUL-S164-TM showed a dramatic increase in the ILA content. Therefore, we inferred that ILA in L. plantarum DPUL-S164-TM plays a key role in improving the intestinal barrier function and alleviating inflammation.

更新日期：2023-11-23

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