<br>细胞壁果胶含量是指叶细胞中带负电的碳点的有利传递,ACS Nano

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细胞壁果胶含量是指叶细胞中带负电的碳点的有利传递
ACS Nano ( IF 15.8 ) Pub Date : 2023-11-22 , DOI: 10.1021/acsnano.3c05182
Lan Zhu _{1,

2} , Wenying Xu ₁ , Xue Yao ₁ , Linlin Chen ₁ , Guangjing Li ₁ , Jiangjiang Gu _{3,

4,

5} , Lu Chen ₃ , Zhaohu Li _{1,

2} , Honghong Wu _{1,

2,

4,

5}

Affiliation

在这项工作中，我们系统地研究了细胞壁和细胞壁成分如何影响带电碳量子点（CD，从-34到+41 mV）向黄瓜和拟南芥植物叶细胞的传递。使用黄瓜和拟南芥中四种不同类型的叶细胞，即原生质体（无细胞壁）、分离的单个细胞（用果胶酶水解细胞壁）、再生单个细胞（由原生质体再生的细胞壁）和完整叶细胞（完整的叶细胞）。细胞壁，在植物中）。叶细胞与带电 CD (0.5 mg/mL) 一起孵育 2 小时。共焦成像结果表明，与 PEI（聚乙烯亚胺）涂覆的带正电的碳点 [PEI ₆₀₀ -CD（17 mV）和PEI _10K -CDs (41 mV)]，而在分离的单个细胞中，趋势相反。细胞壁成分含量结果显示，分离的单个细胞与再生的单个细胞（1.28 vs 1.11 mg/10 ^{6 个}细胞）之间总细胞壁含量没有发现明显变化，而再生的单个细胞则表现出明显更高的果胶含量。含量[水溶性果胶（0.13 vs 0.06 mg/10 ^{6 个}细胞， P < 0.01）、螯合剂可溶性果胶（0.04 vs 0.01 mg/10 ^{6 个}细胞， P < 0.01）和碱性果胶（0.02 vs 0.01 mg） /10 ⁶细胞， P < 0.01)]并且纤维素含量显着低于分离的单个细胞（0.13 vs 0.32 mg/10 ⁶细胞， P < 0.01）。分离的单个细胞和再生的单个细胞之间没有发现半纤维素含量差异（0.20 vs 0.21 mg/10 ^{6 个}细胞）。我们的结果表明，与细胞壁中的纤维素和半纤维素相比，果胶是一个更重要的因素，指的是叶细胞中有利于吸收带负电的碳点。总的来说，这项工作提供了一种研究细胞壁成分在植物细胞吸收纳米颗粒中的作用的方法，并指出了了解细胞屏障和纳米颗粒之间的相互作用对于设计农业用纳米颗粒的重要性。

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Cell Wall Pectin Content Refers to Favored Delivery of Negatively Charged Carbon Dots in Leaf Cells

In this work, we systematically investigated how cell wall and cell wall components affect the delivery of charged carbon quantum dots (CDs, from −34 to +41 mV) to leaf cells of cucumber and Arabidopsis plants. Four different types of leaf cells in cucumber and Arabidopsis were used, i.e., protoplasts (without cell wall), isolated individual cells (cell wall hydrolyzed with pectinase), regenerated individual cells (cell wall regenerated from protoplast), and intact leaf cells (intact cell wall, in planta). Leaf cells were incubated with charged CDs (0.5 mg/mL) for 2 h. Confocal imaging results showed that protoplasts, regenerated individual cells, and leaf cells showed favored uptake of the negatively charged CDs (−34 mV) compared to the PEI (polyethylenimine) coated and positively charged carbon dots [PEI₆₀₀-CDs (17 mV) and PEI_10K-CDs (41 mV)], while in isolated individual cells, the trend is opposite. The results of the content of the cell wall components showed that no significant changes in the total cell wall content were found between isolated individual cells and regenerated individual cells (1.28 vs 1.11 mg/10⁶ cells), while regenerated individual cells showed significant higher pectin content [water-soluble pectin (0.13 vs 0.06 mg/10⁶ cells, P < 0.01), chelator-soluble pectin (0.04 vs 0.01 mg/10⁶ cells, P < 0.01), and alkaline pectin (0.02 vs 0.01 mg/10⁶ cells, P < 0.01)] and significant lower cellulose content (0.13 vs 0.32 mg/10⁶ cells, P < 0.01) than the isolated individual cells. No difference of the hemicellulose content was found between isolated individual cells and regenerated individual cells (0.20 vs 0.21 mg/10⁶ cells). Our results suggest that compared with cellulose and hemicellulose in the cell wall, the pectin is a more important factor referring to the favored uptake of negatively charged carbon dots in leaf cells. Overall, this work provides a method to study the role of cell wall components in the uptake of nanoparticles in plant cells and also points out the importance of understanding the interactions between cell barriers and nanoparticles to design nanoparticles for agricultural use.

更新日期：2023-11-22

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