<br>Qa-SNARE Syntaxin 18 介导脂滴与 SNAP23 和 SEC22B 融合,Cell Discovery

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Qa-SNARE Syntaxin 18 介导脂滴与 SNAP23 和 SEC22B 融合
Cell Discovery ( IF 13.0 ) Pub Date : 2023-11-21 , DOI: 10.1038/s41421-023-00613-4
Yuhui Fu ₁ , Binbin Ding ₂ , Xiaoxia Liu ₁ , Shangang Zhao ₃ , Fang Chen ₁ , Linsen Li ₁ , Yi Zhu ₄ , Jingxuan Zhao ₅ , Zhen Yuan ₂ , Yafeng Shen ₆ , Chaofeng Yang ₇ , Mengle Shao ₈ , She Chen ₉ , Perry E Bickel ₇ , Qing Zhong ₁

Affiliation

Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Department of Pathophysiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Sam and Ann Barshop Institute for Longevity and Aging Studies, Division of Endocrinology and Diabetes, Department of Medicine, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX, USA.
MedChem Service Unit of Shanghai Haoyuan Chemexpress Co., Ltd., Shanghai, China.
Shanghai Institute of Precision of Medicine, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Division of Endocrinology, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX, USA.
CAS Key Laboratory of Molecular Virology and Immunology, The Center for Microbes, Development and Health, Shanghai Institute of Immunity and Infection, Chinese Academy of Sciences, Shanghai, China.
National Institute of Biological Sciences, Beijing, China.

脂滴（LD）是动态的脂质储存细胞器，可以感知并响应全身能量平衡的变化。 LD的大小和数量受到复杂而微妙的机制控制，其中，SNARE蛋白是否介导LD融合以及哪些SNARE蛋白介导LD融合，以及控制这一过程的机制仍知之甚少。在这里，我们鉴定了一个 SNARE 复合体，syntaxin 18 (STX18)–SNAP23–SEC22B，它被招募到 LD 中以介导 LD 融合。 STX18 以 LD 为目标，其跨膜结构域跨越磷脂单层两次。 STX18-SNAP23-SEC22B 复合物在体外脂肪体脂质混合和内容物混合测定中驱动 LD 融合。 CIDEC/FSP27 直接结合 STX18、SEC22B 和 SNAP23，并通过促进 LD 聚类来促进 SNARE 重建脂肪体的脂质混合。在高脂肪饮食条件下，通过 AAV 敲低小鼠肝脏中的 STX18 会导致肝脏变小并减少 LD 大小。所有这些结果都证明了 SNARE 复合体 STX18-SNAP23-SEC22B 在 LD 融合中的关键作用。

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更新日期：2023-11-23

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