A cascade signal-amplified fluorescent biosensor combining APE1 enzyme cleavage-assisted target cycling with rolling circle amplification,Analyst

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A cascade signal-amplified fluorescent biosensor combining APE1 enzyme cleavage-assisted target cycling with rolling circle amplification
Analyst ( IF 3.6 ) Pub Date : 2023-11-21 , DOI: 10.1039/d3an01727c
Zirui Liu ₁ , Hongqun Yang ₁ , Beibei Zhang ₁ , Xinhao Li ₁ , Hong Wang ₁ , Yingwei Zhang ₁

Affiliation

A cascade signal-amplified fluorescent biosensor was developed for miRNA-21 detection by combining APE1 enzyme-assisted target recycling and rolling circle amplification strategy. A key feature of this biosensor is its dual-trigger mechanism, utilizing both tumor-endogenous miRNA-21 and the APE1 enzyme in the initial amplification step, followed by a second rolling circle amplification reaction. This dual signal amplification cascade significantly enhanced sensitivity, achieving a detection limit of 3.33 pM. Furthermore, this biosensor exhibited excellent specificity and resistance to interference, allowing it to effectively distinguish and detect the target miRNA-21 in the presence of multiple interfering miRNAs. Moreover, the biosensor maintained its robust detection capabilities in a 10% serum environment, demonstrating its potential for clinical disease diagnosis applications.

中文翻译：

结合 APE1 酶裂解辅助靶标循环与滚环放大的级联信号放大荧光生物传感器

通过结合 APE1 酶辅助靶标回收和滚环放大策略，开发了用于 miRNA-21 检测的级联信号放大荧光生物传感器。该生物传感器的一个关键特征是其双触发机制，在初始扩增步骤中利用肿瘤内源性 miRNA-21 和 APE1 酶，然后进行第二次滚环扩增反应。这种双信号放大级联显着提高了灵敏度，实现了 3.33 pM 的检测限。此外，该生物传感器表现出优异的特异性和抗干扰性，使其能够在存在多种干扰miRNA的情况下有效区分和检测目标miRNA-21。此外，该生物传感器在10%血清环境中保持了强大的检测能力，展示了其在临床疾病诊断应用中的潜力。

更新日期：2023-11-21

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