2′-OH as a universal handle for studying intracellular RNAs,Cell Chemical Biology

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2′-OH as a universal handle for studying intracellular RNAs
Cell Chemical Biology ( IF 6.6 ) Pub Date : 2023-11-21 , DOI: 10.1016/j.chembiol.2023.10.022
Lu Xiao ₁ , Linglan Fang ₁ , Eric T Kool ₂

Affiliation

RNA plays pivotal roles in most cellular processes, serving as both the traditional carrier of genetic information and as a key regulator of cellular functions. The advent of chemical technologies has contributed critically to the analysis of cellular RNA structures, functions, and interactions. Many of these methods and molecules involve the utilization of chemically reactive handles in RNAs, either introduced externally or inherent within the polymer itself. Among these handles, the 2′-hydroxyl (2′-OH) group has emerged as an exceptionally well-suited and general chemical moiety for the modification and profiling of RNAs in intracellular studies. In this review, we provide an overview of the recent advancements in intracellular applications of acylation at the 2′-OH group of RNA. We outline progress made in probing RNA structure and interactomes, controlling RNA function, RNA imaging, and analyzing RNA-small molecule interactions, all achieved in living cells through this simple chemical handle on the biopolymer.

中文翻译：

2′-OH 作为研究细胞内 RNA 的通用句柄

RNA 在大多数细胞过程中发挥着关键作用，既是遗传信息的传统载体，又是细胞功能的关键调节剂。化学技术的出现对细胞 RNA 结构、功能和相互作用的分析做出了重要贡献。许多这些方法和分子涉及RNA中化学反应性手柄的利用，这些化学反应性手柄要么是外部引入的，要么是聚合物本身固有的。在这些手柄中，2′-羟基 (2′-OH) 基团已成为一种非常适合细胞内研究中 RNA 修饰和分析的通用化学部分。在这篇综述中，我们概述了 RNA 2'-OH 基团酰化在细胞内应用的最新进展。我们概述了在探测 RNA 结构和相互作用组、控制 RNA 功能、RNA 成像以及分析 RNA-小分子相互作用方面取得的进展，所有这些都通过生物聚合物上的这种简单化学处理在活细胞中实现。

更新日期：2023-11-21

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