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Dual-channel fluorescent probe for discriminative detection of H2S and N2H4: Exploring sensing mechanism and real-time applications
Journal of Hazardous Materials ( IF 12.2 ) Pub Date : 2023-11-22 , DOI: 10.1016/j.jhazmat.2023.133036 Kanagaraj Rajalakshmi 1 , Selvaraj Muthusamy 1 , Ho-Jin Lee 2 , Palanisamy Kannan 3 , Dongwei Zhu 1 , Jong-Won Song 4 , Yun-Sik Nam 5 , Dong Nyoung Heo 6 , Il Keun Kwon 6 , Zhibin Luo 1 , Yuanguo Xu 1
Journal of Hazardous Materials ( IF 12.2 ) Pub Date : 2023-11-22 , DOI: 10.1016/j.jhazmat.2023.133036 Kanagaraj Rajalakshmi 1 , Selvaraj Muthusamy 1 , Ho-Jin Lee 2 , Palanisamy Kannan 3 , Dongwei Zhu 1 , Jong-Won Song 4 , Yun-Sik Nam 5 , Dong Nyoung Heo 6 , Il Keun Kwon 6 , Zhibin Luo 1 , Yuanguo Xu 1
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A highly efficient system incorporates the real-time visualization of the two toxic molecules (H2 S and N2 H4 ) and the recognition of corresponding transforms using a fluorescent sensor. In this paper, a dual-responsive probe (QS-DNP ) based on methylquinolinium-salicyaldehyde-2,4-dinitrophenyl was developed that can simultaneously detect H2 S and N2 H4 at two independent fluorescent channels without signal crosstalk. QS-DNP showed excellent anti-interference, high selectivity, outstanding water solubility, low LOD values (H2 S: 51 nM; N2 H4 : 40 nM), low cytotoxicity, and mitochondrial localization properties. The 2,4-dinitrophenyl site was sensitive to H2 S, and the C2 H4 , with strong fluorescence at 680 and 488 nm, respectively. The wavelength gap between these two channels is 192 nm; verify that there is no signal crosstalk throughout detection. By this means, the probe was used to simultaneously detect H2 S and N2 H4 in real soil samples, food samples, and living cells. The endogenous H2 S and N2 H4 were monitored in HeLa cells and investigated the mitochondria organelle of living cells with a positive charge on QS-DNP . Overall, all results emphasize that the QS-DNP probe is a powerful tool for the simultaneous detection of H2 S and N2 H4 and presents a potential new sensing approach.
中文翻译：
用于 H2S 和 N2H4 鉴别检测的双通道荧光探针：探索传感机制和实时应用
一个高效的系统结合了两种有毒分子（H2S 和 N2H4）的实时可视化以及使用荧光传感器识别相应的转化。本文开发了一种基于甲基喹啉-水杨醛-2,4-二硝基苯基的双响应探针 （QS-DNP），该探针可以在两个独立的荧光通道上同时检测 H2S 和 N2H4，而不会产生信号串扰。QS-DNP表现出优异的抗干扰性、高选择性、出色的水溶性、低LOD值（H2S：51 nM;N2H4：40 nM）、低细胞毒性和线粒体定位特性。2,4-二硝基苯基位点对 H2S 敏感，CC 桥对 N2H4 具有反应性，分别在 680 和 488 nm 处具有强荧光。这两个通道之间的波长间隙为 192 nm;验证在整个检测过程中没有信号串扰。通过这种方式，该探针用于同时检测真实土壤样品、食品样品和活细胞中的 H2S 和 N2H4。监测 HeLa 细胞中的内源性 H2S 和 N2H4，并研究对 QS-DNP 带正电荷的活细胞的线粒体细胞器。总体而言，所有结果都强调 QS-DNP 探针是同时检测 H2S 和 N2H4 的有力工具，并提出了一种潜在的新传感方法。
更新日期：2023-11-22
中文翻译：
用于 H2S 和 N2H4 鉴别检测的双通道荧光探针：探索传感机制和实时应用
一个高效的系统结合了两种有毒分子（H2S 和 N2H4）的实时可视化以及使用荧光传感器识别相应的转化。本文开发了一种基于甲基喹啉-水杨醛-2,4-二硝基苯基的双响应探针 （QS-DNP），该探针可以在两个独立的荧光通道上同时检测 H2S 和 N2H4，而不会产生信号串扰。QS-DNP表现出优异的抗干扰性、高选择性、出色的水溶性、低LOD值（H2S：51 nM;N2H4：40 nM）、低细胞毒性和线粒体定位特性。2,4-二硝基苯基位点对 H2S 敏感，CC 桥对 N2H4 具有反应性，分别在 680 和 488 nm 处具有强荧光。这两个通道之间的波长间隙为 192 nm;验证在整个检测过程中没有信号串扰。通过这种方式，该探针用于同时检测真实土壤样品、食品样品和活细胞中的 H2S 和 N2H4。监测 HeLa 细胞中的内源性 H2S 和 N2H4，并研究对 QS-DNP 带正电荷的活细胞的线粒体细胞器。总体而言，所有结果都强调 QS-DNP 探针是同时检测 H2S 和 N2H4 的有力工具，并提出了一种潜在的新传感方法。
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