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Dual-channel fluorescent probe for discriminative detection of H2S and N2H4: Exploring sensing mechanism and real-time applications
Journal of Hazardous Materials ( IF 12.2 ) Pub Date : 2023-11-22 , DOI: 10.1016/j.jhazmat.2023.133036 Kanagaraj Rajalakshmi 1 , Selvaraj Muthusamy 1 , Ho-Jin Lee 2 , Palanisamy Kannan 3 , Dongwei Zhu 1 , Jong-Won Song 4 , Yun-Sik Nam 5 , Dong Nyoung Heo 6 , Il Keun Kwon 6 , Zhibin Luo 1 , Yuanguo Xu 1
Journal of Hazardous Materials ( IF 12.2 ) Pub Date : 2023-11-22 , DOI: 10.1016/j.jhazmat.2023.133036 Kanagaraj Rajalakshmi 1 , Selvaraj Muthusamy 1 , Ho-Jin Lee 2 , Palanisamy Kannan 3 , Dongwei Zhu 1 , Jong-Won Song 4 , Yun-Sik Nam 5 , Dong Nyoung Heo 6 , Il Keun Kwon 6 , Zhibin Luo 1 , Yuanguo Xu 1
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A highly efficient system incorporates the real-time visualization of the two toxic molecules (HS and NH) and the recognition of corresponding transforms using a fluorescent sensor. In this paper, a dual-responsive probe () based on methylquinolinium-salicyaldehyde-2,4-dinitrophenyl was developed that can simultaneously detect HS and NH at two independent fluorescent channels without signal crosstalk. showed excellent anti-interference, high selectivity, outstanding water solubility, low LOD values (HS: 51 nM; NH: 40 nM), low cytotoxicity, and mitochondrial localization properties. The 2,4-dinitrophenyl site was sensitive to HS, and the CC bridge was reactive to NH, with strong fluorescence at 680 and 488 nm, respectively. The wavelength gap between these two channels is 192 nm; verify that there is no signal crosstalk throughout detection. By this means, the probe was used to simultaneously detect HS and NH in real soil samples, food samples, and living cells. The endogenous HS and NH were monitored in HeLa cells and investigated the mitochondria organelle of living cells with a positive charge on . Overall, all results emphasize that the probe is a powerful tool for the simultaneous detection of HS and NH and presents a potential new sensing approach.
中文翻译：
用于区分检测 H2S 和 N2H4 的双通道荧光探针：探索传感机制和实时应用
高效系统结合了两种有毒分子（H2S 和 NH）的实时可视化以及使用荧光传感器识别相应变换。本文开发了一种基于甲基喹啉鎓-水杨醛-2,4-二硝基苯基的双响应探针（），可以在两个独立的荧光通道上同时检测HS和NH，而不会出现信号串扰。表现出优异的抗干扰性、高选择性、出色的水溶性、低LOD值（HS：51 nM；NH：40 nM）、低细胞毒性和线粒体定位特性。 2,4-二硝基苯基位点对H2S敏感，CC桥对NH2反应，分别在680和488 nm处发出强荧光。这两个通道之间的波长差距为192 nm；验证整个检测过程中没有信号串扰。通过这种方式，该探针可同时检测真实土壤样品、食品样品和活细胞中的H2S和NH。在 HeLa 细胞中监测内源 HS 和 NH，并研究带有正电荷的活细胞的线粒体细胞器。总体而言，所有结果都强调该探针是同时检测 H2S 和 NH 的强大工具，并提出了一种潜在的新传感方法。
更新日期：2023-11-22
中文翻译：
用于区分检测 H2S 和 N2H4 的双通道荧光探针：探索传感机制和实时应用
高效系统结合了两种有毒分子（H2S 和 NH）的实时可视化以及使用荧光传感器识别相应变换。本文开发了一种基于甲基喹啉鎓-水杨醛-2,4-二硝基苯基的双响应探针（），可以在两个独立的荧光通道上同时检测HS和NH，而不会出现信号串扰。表现出优异的抗干扰性、高选择性、出色的水溶性、低LOD值（HS：51 nM；NH：40 nM）、低细胞毒性和线粒体定位特性。 2,4-二硝基苯基位点对H2S敏感，CC桥对NH2反应，分别在680和488 nm处发出强荧光。这两个通道之间的波长差距为192 nm；验证整个检测过程中没有信号串扰。通过这种方式，该探针可同时检测真实土壤样品、食品样品和活细胞中的H2S和NH。在 HeLa 细胞中监测内源 HS 和 NH，并研究带有正电荷的活细胞的线粒体细胞器。总体而言，所有结果都强调该探针是同时检测 H2S 和 NH 的强大工具，并提出了一种潜在的新传感方法。
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