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Tumor-Targeted Fluorescent/Photoacoustic Imaging of Legumain Activity In Vivo
ACS Sensors ( IF 8.2 ) Pub Date : 2023-11-20 , DOI: 10.1021/acssensors.3c01922 Minghui Wang 1 , Menglin Tao 1 , Wujuan Zhu 1 , Wenbin Liu 1 , Zhengjie Liu 1 , Zijuan Hai 1
ACS Sensors ( IF 8.2 ) Pub Date : 2023-11-20 , DOI: 10.1021/acssensors.3c01922 Minghui Wang 1 , Menglin Tao 1 , Wujuan Zhu 1 , Wenbin Liu 1 , Zhengjie Liu 1 , Zijuan Hai 1
Affiliation
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Legumain has been identified as a target for diagnosis and treatment of associated cancers. Therefore, real-time imaging of legumain activity in vivo is helpful in diagnosing and evaluating therapeutic efficacy of associated cancers. Fluorescent/photoacoustic (FL/PA) dual-modal imaging developed rapidly because of its good sensitivity and spatial resolution. As far as we know, a tumor-targeted probe for FL/PA imaging of legumain activity in vivo has not been reported. Hence, we intended to develop a tumor-targeted hemicyanine (HCy) probe (HCy-AAN-Bio) for FL/PA imaging of legumain in vivo. The control probe HCy-AAN does not have tumor-targeting ability. Legumain can specifically cleave HCy-AAN-Bio or HCy-AAN with the generation of FL/PA signal while more HCy-AAN-Bio could be recognized by legumain than HCy-AAN with higher sensitivity in vitro. Due to the tumor-targeting ability, HCy-AAN-Bio could image 4T1 cells with an additional 1.3-fold FL enhancement and 1.9-fold PA enhancement than HCy-AAN. In addition, HCy-AAN-Bio could image legumain activity in vivo with an additional 1.5-fold FL enhancement and 1.9-fold PA enhancement than HCy-AAN. We expected that HCy-AAN-Bio will be a powerful tool for early diagnosis of associated cancer.
中文翻译：
Legumain 体内活性的肿瘤靶向荧光/光声成像
Legumain 已被确定为诊断和治疗相关癌症的靶点。因此，体内legumain活性的实时成像有助于诊断和评估相关癌症的治疗效果。荧光/光声（FL/PA）双模态成像因其良好的灵敏度和空间分辨率而迅速发展。据我们所知，用于体内legumain活性FL/PA成像的肿瘤靶向探针尚未见报道。因此，我们打算开发一种肿瘤靶向半花青（HCy）探针（ HCy-AAN-Bio ），用于legumain体内FL/PA成像。对照探针HCy-AAN不具有肿瘤靶向能力。 Legumain 可以特异性裂解HCy-AAN-Bio或HCy-AAN ，并产生 FL/PA 信号，而在体外，legumain 比HCy-AAN能识别更多的HCy-AAN-Bio ，且灵敏度更高。由于具有肿瘤靶向能力， HCy-AAN-Bio对 4T1 细胞的成像比HCy-AAN具有额外 1.3 倍的 FL 增强和 1.9 倍的 PA 增强。此外， HCy-AAN-Bio可以对 legumain 体内活性进行成像，与HCy-AAN相比，FL 增强 1.5 倍，PA 增强 1.9 倍。我们预计HCy-AAN-Bio将成为相关癌症早期诊断的强大工具。
更新日期：2023-11-20
中文翻译：
Legumain 体内活性的肿瘤靶向荧光/光声成像
Legumain 已被确定为诊断和治疗相关癌症的靶点。因此，体内legumain活性的实时成像有助于诊断和评估相关癌症的治疗效果。荧光/光声（FL/PA）双模态成像因其良好的灵敏度和空间分辨率而迅速发展。据我们所知，用于体内legumain活性FL/PA成像的肿瘤靶向探针尚未见报道。因此，我们打算开发一种肿瘤靶向半花青（HCy）探针（ HCy-AAN-Bio ），用于legumain体内FL/PA成像。对照探针HCy-AAN不具有肿瘤靶向能力。 Legumain 可以特异性裂解HCy-AAN-Bio或HCy-AAN ，并产生 FL/PA 信号，而在体外，legumain 比HCy-AAN能识别更多的HCy-AAN-Bio ，且灵敏度更高。由于具有肿瘤靶向能力， HCy-AAN-Bio对 4T1 细胞的成像比HCy-AAN具有额外 1.3 倍的 FL 增强和 1.9 倍的 PA 增强。此外， HCy-AAN-Bio可以对 legumain 体内活性进行成像，与HCy-AAN相比，FL 增强 1.5 倍，PA 增强 1.9 倍。我们预计HCy-AAN-Bio将成为相关癌症早期诊断的强大工具。
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