Gas Vesicle–Blood Interactions Enhance Ultrasound Imaging Contrast,Nano Letters

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Gas Vesicle–Blood Interactions Enhance Ultrasound Imaging Contrast
Nano Letters ( IF 9.6 ) Pub Date : 2023-11-20 , DOI: 10.1021/acs.nanolett.3c02780
Bill Ling ₁ , Jeong Hoon Ko ₁ , Benjamin Stordy _{2,

3} , Yuwei Zhang _{2,

3,

4} , Tighe F Didden ₁ , Dina Malounda ₁ , Margaret B Swift ₁ , Warren C W Chan _{2,

3,

4} , Mikhail G Shapiro _{1,

5,

6}

Affiliation

Gas vesicles (GVs) are genetically encoded, air-filled protein nanostructures of broad interest for biomedical research and clinical applications, acting as imaging and therapeutic agents for ultrasound, magnetic resonance, and optical techniques. However, the biomedical applications of GVs as systemically injectable nanomaterials have been hindered by a lack of understanding of GVs’ interactions with blood components, which can significantly impact in vivo behavior. Here, we investigate the dynamics of GVs in the bloodstream using a combination of ultrasound and optical imaging, surface functionalization, flow cytometry, and mass spectrometry. We find that erythrocytes and serum proteins bind to GVs and shape their acoustic response, circulation time, and immunogenicity. We show that by modifying the GV surface we can alter these interactions and thereby modify GVs’ in vivo performance. These results provide critical insights for the development of GVs as agents for nanomedicine.

中文翻译：

气体囊泡-血液相互作用增强超声成像对比度

气体囊泡（GV）是基因编码的、充满空气的蛋白质纳米结构，在生物医学研究和临床应用中具有广泛的意义，可作为超声、磁共振和光学技术的成像和治疗剂。然而，由于缺乏对 GV 与血液成分相互作用的了解，GV 作为可全身注射的纳米材料的生物医学应用受到阻碍，这种相互作用会显着影响体内行为。在这里，我们结合超声和光学成像、表面功能化、流式细胞术和质谱法研究了血流中 GV 的动态。我们发现红细胞和血清蛋白与 GV 结合并影响其声学反应、循环时间和免疫原性。我们表明，通过修改 GV 表面，我们可以改变这些相互作用，从而改变 GV 的体内性能。这些结果为 GV 作为纳米医学制剂的开发提供了重要的见解。

更新日期：2023-11-20

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