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Droplet manipulation on an adjustable closed-open digital microfluidic system utilizing asymmetric EWOD
Lab on a Chip ( IF 6.1 ) Pub Date : 2023-11-20 , DOI: 10.1039/d3lc00856h Jingsong Xu 1 , Xingcheng Wang 1 , Qingyuan Huang 1 , Xiaodong He 1
Lab on a Chip ( IF 6.1 ) Pub Date : 2023-11-20 , DOI: 10.1039/d3lc00856h Jingsong Xu 1 , Xingcheng Wang 1 , Qingyuan Huang 1 , Xiaodong He 1
Affiliation
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The closed-open digital microfluidic (DMF) system offers a versatile and powerful platform for various applications by combining the advantages of both closed and open structures. The current closed-open DMF system faces challenges in scaling up due to electrode structural differences between closed and open regions. Here we developed an adjustable closed-open DMF platform by utilizing the modified slippery liquid-infused porous surfaces (SLIPS) with asymmetric electrowetting on dielectric (AEWOD) as a hydrophobic dielectric layer. The consistent electrode structures of the bottom printed circuit board (PCB) electrode array on both the closed and open regions, and the utilization of a transparent acrylic with floating potential as the top plate allow a low-cost and easily scalable closed-open DMF system to be achieved. The impacts of applied voltage, parallel plate spacing, electrode switching interval, and electrode driving strategies on various droplet manipulations were investigated. The results show that the optimal plate spacings range from 340–510 μm within the closed region. Meanwhile, we also studied the influence of the thickness, geometry, and position of the top plate on the droplet movement at the closed-open boundary. Through force analysis and experimentation, it is found that a thin top plate and a bevel of ∼4° can effectively facilitate the movement of droplets at the boundary. Finally, we successfully achieved protein staining experiments on this platform and developed a customized smartphone application for the accurate detection of protein concentration. This innovative closed-open DMF system provides new possibilities for future applications in real-time biological sample processing and detection.
中文翻译：
利用非对称 EWOD 对可调节闭开数字微流体系统进行液滴操作
封闭式数字微流控（DMF）系统结合了封闭式和开放式结构的优点，为各种应用提供了一个多功能且强大的平台。由于封闭区域和开放区域之间的电极结构差异，当前的封闭-开放 DMF 系统在扩大规模方面面临着挑战。在这里，我们利用改性的光滑液体注入多孔表面（SLIPS）和不对称电润湿电介质（AEWOD）作为疏水介电层，开发了一种可调节的闭开式DMF平台。底部印刷电路板 (PCB) 电极阵列在闭合区域和开放区域上的一致电极结构，以及使用具有浮动电位的透明丙烯酸作为顶板，可实现低成本且易于扩展的闭开 DMF 系统要实现的。研究了施加电压、平行板间距、电极切换间隔和电极驱动策略对各种液滴操作的影响。结果表明，封闭区域内最佳板间距范围为 340–510 μm。同时，我们还研究了顶板的厚度、几何形状和位置对闭-开边界处液滴运动的影响。通过受力分析和实验发现，较薄的顶板和~4°的斜角可以有效促进液滴在边界处的运动。最终，我们在该平台上成功实现了蛋白质染色实验，并开发了定制的智能手机应用程序，用于准确检测蛋白质浓度。这种创新的闭开式DMF系统为未来在实时生物样品处理和检测方面的应用提供了新的可能性。
更新日期：2023-11-20
中文翻译：
利用非对称 EWOD 对可调节闭开数字微流体系统进行液滴操作
封闭式数字微流控（DMF）系统结合了封闭式和开放式结构的优点，为各种应用提供了一个多功能且强大的平台。由于封闭区域和开放区域之间的电极结构差异，当前的封闭-开放 DMF 系统在扩大规模方面面临着挑战。在这里，我们利用改性的光滑液体注入多孔表面（SLIPS）和不对称电润湿电介质（AEWOD）作为疏水介电层，开发了一种可调节的闭开式DMF平台。底部印刷电路板 (PCB) 电极阵列在闭合区域和开放区域上的一致电极结构，以及使用具有浮动电位的透明丙烯酸作为顶板，可实现低成本且易于扩展的闭开 DMF 系统要实现的。研究了施加电压、平行板间距、电极切换间隔和电极驱动策略对各种液滴操作的影响。结果表明，封闭区域内最佳板间距范围为 340–510 μm。同时，我们还研究了顶板的厚度、几何形状和位置对闭-开边界处液滴运动的影响。通过受力分析和实验发现，较薄的顶板和~4°的斜角可以有效促进液滴在边界处的运动。最终，我们在该平台上成功实现了蛋白质染色实验，并开发了定制的智能手机应用程序，用于准确检测蛋白质浓度。这种创新的闭开式DMF系统为未来在实时生物样品处理和检测方面的应用提供了新的可能性。
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