大肠杆菌生产水杨酸2-O-β-d-葡萄糖苷的代谢工程[J].,Chinese Journal of Biotechnology

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大肠杆菌生产水杨酸2-O-β-d-葡萄糖苷的代谢工程[J].
Chinese Journal of Biotechnology Pub Date : 2023-08-25 , DOI: 10.13345/j.cjb.230264
Ruosong Li _{1,

2,

3} , Yanfeng Peng ₂ , Long Ma _{1,

3} , Qinhong Wang ₂

Affiliation

水杨酸2-O-β-d-葡萄糖苷（SAG）是植物中水杨酸的衍生物。最近的报道表明，SAG由于具有抗炎和镇痛作用，并且与水杨酸和阿司匹林相比刺激性较小，因此可以被认为是一种潜在的抗炎物质。生物法利用可再生资源生产水杨酸化合物，比传统工业方法更加环保。本研究以大肠杆菌Tyr002为起始菌株，通过引入铜绿假单胞菌的异分支酸丙酮酸裂解酶基因pchB，构建了大肠杆菌产水杨酸菌株。通过调控下游芳香氨基酸代谢途径关键基因的表达，摇瓶发酵水杨酸滴度达到1.05 g/L。随后，将外源水杨酸糖基转移酶引入水杨酸生产菌株中以糖基化水杨酸。新工程菌株在摇瓶发酵中产生 5.7 g/L SAG。在随后的5L发酵罐分批补料发酵中，SAG的滴度达到36.5g/L，这是迄今为止报道的最高滴度。该工作为水杨酸盐及其衍生物的生物合成提供了一条新途径。

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[Metabolic engineering of Escherichia coli for production of salicylate 2-O-β-d-glucoside].

Salicylate 2-O-β-d-glucoside (SAG) is a derivative of salicylate in plants. Recent reports showed that SAG could be considered as a potential anti-inflammatory substance due to its anti-inflammatory and analgesic effects, and less irritation compared with salicylic acid and aspirin. The biological method uses renewable resources to produce salicylic acid compounds, which is more environmentally friendly than traditional industry methods. In this study, Escherichia coli Tyr002 was used as the starting strain, and a salicylic acid producing strain of E. coli was constructed by introducing the isochorismate pyruvate lyase gene pchB from Pseudomonas aeruginosa. By regulating the expression of the key genes in the downstream aromatic amino acid metabolic pathways, the titer of salicylic acid reached 1.05 g/L in shake flask fermentation. Subsequently, an exogenous salicylic acid glycosyltransferase was introduced into the salicylic acid producing strain to glycosylate the salicylic acid. The newly engineered strain produced 5.7 g/L SAG in shake flask fermentation. In the subsequent batch fed fermentation in a 5 L fermentation tank, the titer of SAG reached 36.5 g/L, which is the highest titer reported to date. This work provides a new route for biosynthesis of salicylate and its derivatives.

更新日期：2023-08-25

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