Magnetic anastomosis substantially shortens the duration of vascular anastomosis. We aimed to apply magnetic anastomosis technology (MAT) to donor liver implantations in pig orthotopic liver transplantation (OLT). Twenty healthy adult pigs were randomly divided into donors and recipients, and major vascular anastomosis was performed using MAT during OLT. Recipient liver and kidney function was measured pre-surgery and 12, 24 and 72 h post-surgery. Vascular anastomoses examinations were performed using ultrasound or angiography weekly post-surgery, and pathological examinations of vascular anastomoses were performed during autopsy after animal euthanasia. All recipients survived 24 h after surgery, which is considered as successful transplantation. Anhepatic duration was only 13 min, and no anastomotic obstruction or stenosis, magnetic displacement and anastomotic angulation, or distortion was found upon postoperative examinations of major liver vasculature. Aspartate aminotransferase, alanine aminotransferase, and total bilirubin serum levels increased considerably postoperatively. The follow-up period for this study was 1 year, and the median survival time of all recipients was 115 d (interquartile range = 11–180 d). The main causes of death were liver failure, immune rejection, infection, and arterial anastomotic bleeding. Moreover, vascular anastomoses healed well with a survival time of more than two weeks. We developed a novel magnetic device to create a fast and safe technique to perform major vascular anastomoses in pig liver transplantations. Additionally, the liver graft implantation using MAT considerably shortened the recipient warm ischemia time, which will reduce the extent of ischemia–reperfusion injury. We conclude that MAT is an effective method for donor liver fast implantation in OLT in pigs.

磁力吻合大大缩短了血管吻合的时间。我们的目标是将磁吻合技术（MAT）应用于猪原位肝移植（OLT）中的供体肝脏植入。将20头健康成年猪随机分为供体和受体，OLT时采用MAT进行大血管吻合。术前以及术后 12、24 和 72 小时测量受者的肝肾功能。术后每周使用超声或血管造影进行血管吻合检查，并在动物安乐死后尸检时进行血管吻合的病理检查。术后24小时，所有受者均存活，即视为移植成功。无肝时间仅13分钟，术后肝大血管检查未发现吻合口梗阻或狭窄、磁位移和吻合口成角或扭曲。术后血清天冬氨酸转氨酶、丙氨酸转氨酶和总胆红素水平显着升高。本研究的随访期为1年，所有受者的中位生存时间为115天（四分位距=11-180天）。死亡的主要原因是肝功能衰竭、免疫排斥、感染和动脉吻合口出血。此外，血管吻合口愈合良好，存活时间超过两周。我们开发了一种新型磁性装置，以创建一种快速、安全的技术来在猪肝移植中进行主要血管吻合。此外，使用MAT进行的肝移植大大缩短了受者的热缺血时间，从而减轻了缺血再灌注损伤的程度。 我们得出的结论是，MAT 是供体肝脏快速植入猪 OLT 的有效方法。