Yellow vein mosaic virus (YVMV) poses a significant threat to okra production. Breeding for genetic resistance is a promising approach for YVMV management. This study aimed to investigate the inheritance of YVMV resistance and identify molecular markers associated with resistance in okra. Recombinant inbred lines (RILs) were developed from a cross between a YVMV-resistant breeding line (NS25581) and a susceptible line (NS17556). Phenotypic data from the RIL population exhibited a segregation ratio of 1:3 for resistant and susceptible plants, suggesting the involvement of a single recessive gene in controlling YVMV resistance over two consecutive years (Summer 2020 and 2021). A parental polymorphism survey using 212 markers identified six polymorphic markers. Genotyping of RILs with these markers, followed by single marker analysis, revealed a significant association between YVMV resistance and the SSR54 marker. SSR54 accounted for 4.42% and 5.05% of the phenotypic variance (R²) during Summer 2020 and Summer 2021, respectively. Notably, this study marks the first identification of a marker associated with YVMV resistance in okra. These findings provide valuable insights into the genetic basis of YVMV resistance and pave the way for marker-assisted breeding programs targeting enhanced resistance in okra cultivars.

黄脉花叶病毒（YVMV）对秋葵生产构成重大威胁。遗传抗性育种是 YVMV 管理的一种有前景的方法。本研究旨在研究黄秋葵 YVMV 抗性的遗传并鉴定与抗性相关的分子标记。重组自交系 (RIL) 是由 YVMV 抗性育种系 (NS25581) 和易感系 (NS17556) 杂交开发而成。RIL 群体的表型数据显示，抗性和易感植物的分离比为 1:3，表明单个隐性基因连续两年（2020 年夏季和 2021 年夏季）参与控制 YVMV 抗性。使用 212 个标记进行的亲本多态性调查鉴定出 6 个多态性标记。使用这些标记对 RIL 进行基因分型，然后进行单一标记分析，揭示了 YVMV 抗性与 SSR54 标记之间的显着关联。SSR54分别占2020年夏季和2021年夏季表型变异（R ^{2 ）的4.42%和5.05%。}值得注意的是，这项研究标志着首次鉴定出与秋葵中 YVMV 抗性相关的标记。这些发现为了解 YVMV 抗性的遗传基础提供了宝贵的见解，并为针对增强秋葵品种抗性的标记辅助育种计划铺平了道路。